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Table 3 The estimated minimal sample number per group for five proteins based on western blot, where power is the power of statistical test, α is the significance level, and π refers to the estimated proportion of truly differentially expressed proteins among all of the identified proteins.

From: An individual urinary proteome analysis in normal human beings to define the minimal sample number to represent the normal urinary proteome

fold change

power

α

π

FDR

alpha 1

ceruloplasmin

beta-2

     

antitrypsin

 

microglobulin

2

0.8

0.001

0.05

2.31%

26

38

33

 

0.8

0.005

0.1

5.33%

20

30

26

 

0.8

0.01

0.2

4.76%

18

26

22

 

0.9

0.001

0.05

2.06%

31

46

39

 

0.9

0.005

0.1

4.76%

25

37

31

 

0.9

0.01

0.2

4.26%

22

33

28

1.5

0.8

0.001

0.05

2.31%

93

143

121

 

0.8

0.005

0.1

5.33%

73

111

95

 

0.8

0.01

0.2

4.76%

64

98

83

 

0.9

0.001

0.05

2.06%

113

174

148

 

0.9

0.005

0.1

4.76%

91

139

118

 

0.9

0.01

0.2

4.26%

81

124

105