Table 3 Oligonucleotide primers for real-time RT PCR
Spot | Protein | Gene | Accession No. a | Forward primer (5’–3’) | Reverse primer (5’–3’) | Size (bp) |
|---|---|---|---|---|---|---|
104 | ATPase β subunit | atpB | AY300043 | ATGAGAGTTGGTTTGACTGC | CGAATTGTTTCTGCTAGACC | 629 |
304 | Rubisco (large subunit) | rbcL | M76402 | GAACGTGAACTCACAACCAT | GACATACGTAACGCTTTTGC | 351 |
172 | Rubisco activase | rca | SGN-U243405 | AATACACCGTCAACAACCAG | CACCAATGTTTTCAATTCCA | 382 |
349 | l-Ascorbate peroxidase (chloroplastic) | apx | SGN-U247328 + SGN-U258329 | ATGAGGATCGCTTTCATAGAC | ATTTTCTGGTCTGCTGATCTC | 311 |
444 | PsaD (PSI subunit II) | psaD | STU556864 | TGGAAACAATCCCTCCTATC | ACAAATTGGGTCCTTCTCTC | 310 |
451 | PsaE-B (PSI subunit IV-B) | psaE-B | SGN-U245041 | CCTAATGTCACCTCTAACTCTG | TAAAACATGGAAAGCACAGG | 458 |
--- | Proteinase inhibitor II | pinII | L37519 | AATCTTGGGTTTGGGATATG | TATGTGGATCGCAATTTAGG | 178 |
--- | PR-1 protein P4 | p4 | AJ250136 | GCACAAAATTATGCCAACTC | AGTTGCATGAAATGAACCAC | 271 |
--- | Elongation factor 1-a | ef1-α | AB061263 | ATTGGAAACGGATATGCTCCA | TCCTTACCTGAACGCCTGTCA | 101 |