Figure 3
From: Proteomic analysis of S-nitrosylation induced by 1-methyl-4-phenylpyridinium (MPP+)
Detection of S-nitrosylated ESD, STRAP, TCP-1 γ , and ERp46. ESD (A), STRAP (B), TCP-1 γ (C), and ERp46 (D) were overexpressed in SH-SY5Y cells, and lysates prepared from the treated cells were analyzed by the SNO-RAC assay, in which ascorbate-dependent capture of proteins by resin indicates the presence of S-nitrosylated cysteine residues. Elutants were subjected to immunoblot analysis using anti-FLAG antibody.