Figure 4

S-nitrosylation of ESD, STRAP, TCP-1 γ , and ERp46 in neuroblastoma cells treated with MPP⁺ . ESD (A), STRAP (B), TCP-1 γ (C), and ERp46 (D) were overexpressed in SH-SY5Y cells. The SH-SY5Y cells were then treated with MPP⁺ (100 μM) for 3 h, and lysates prepared from the treated cells were analyzed by the SNO-RAC assay, in which ascorbate-dependent capture of proteins by resin indicates the presence of S-nitrosylated cysteine residues. Elutants were subjected to immunoblot analysis using anti-FLAG antibody.