Pre-analytical stability of the plasma proteomes based on the storage temperature

Table 1 Plasma protein spots identified by MALDI-TOF MS

Spot	Identified protein	Acc. no. ^a	Score ^b	QM ^c	% C ^d	Theoretical M r/pI^e	Observed M r/pI^f
		ALB/AFP/VDB family
1	Vitamin D-binding protein	P02774	100	11	43	52964/5.4	55700/5.3
2	Serum albumin	P02768	95	12	28	69367/5.92	67600/5.4
3	Serum albumin	P02768	142	18	41	69367/5.92	57000/5.4
4	Serum albumin	P02768	224	21	43	69367/5.92	56900/5.6
		Serpin family
5	Alpha-1-antitrypsin	P01009	133	17	34	46737/5.37	56000/5.0
6	Alpha-1-antitrypsin	P01009	117	10	32	46737/5.37	56100/5.1
		Fibrinogen alpha/beta family
7	Fibrinogen beta chain	P02675	41	7	20	55928/8.54	55700/6.3
8	Fibrinogen beta chain	P02675	95	10	27	55928/8.54	55300/6.4
9	Fibrinogen gamma chain	P02679	177	14	43	51512/5.37	51500/5.7
10	Fibrinogen gamma chain	P02679	177	14	45	51512/5.37	51700/5.3
11	Fibrinogen gamma chain	P02679	164	14	39	51512/5.37	51200/5.6
12	Fibrinogen gamma chain	P02679	177	14	43	51512/5.37	51700/5.5
		Transferrin family
13	Serotransferrin	P02787	423	40	54	77064/6.81	57200/6.4
14	Serotransferrin	P02787	81	10	20	77064/6.81	57300/6.3
15	Serotransferrin	P02787	196	20	31	77064/6.81	57400/6.4
		Apolipoprotein AI/A4/E family
16	Apolipoprotein A-I	P02647	60	7	28	30778/5.56	23000/5.2
17	Apolipoprotein-E	P02649	151	17	55	36154/5.65	33500/5.6
		Peptidase S1 family
18	Haptoglobin	P00738	64	9	19	45205/6.13	45300/5.0
19	Haptoglobin	P00738	70	10	25	45205/6.13	42800/5.5
20	Complement factor B	P00751	60	5	13	85533/6.67	97900/6.2

^a Accession number (database: UniProtKB/Swiss-Prot).
^b Score: probability score in MASCOT program (the probability that the observed match between the experimental data and mass values calculated from a candidate peptide sequence is a random event).
^c Queries matched indicates the number of matched peptides in the database search.
^d Percent coverage: the minimum coverage of the matched peptide in relation to the full-length sequence.
^e Theoretical molecular weight/isoelectric point of full-length protein according to the Swiss-Prot database.
^f Molecular weight/isoelectric point of full-length protein calculated upon calibration of electrophoretic gels.

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