Skip to main content

Table 4 comparison of malto-oligosaccharide soluble and insoluble populations

From: The 3T3-L1 adipocyte glycogen proteome

Name

ID

Sol

Insol

Sol/insol

Compartment

Glycogen-branching enzyme

GLGB_MOUSE

0.25

0.03

7.39

g

Glycogen debranching enzyme *

GDE_HUMAN

0.04

0.02

1.73

g

Protein phosphatase 1 regulatory subunit 3D

A2AJW4_MOUSE

0.03

0.03

1.13

g

Glycogen phosphorylase, brain form

PYGB_MOUSE

1.00

1.00

1.00

g

Pyruvate carboxylase

PYC_MOUSE

0.02

0.05

0.46

m

Histone H4 †

H4_MOUSE

0.21

0.47

0.44

n

Histone H2B type 1-F/J/L †

H2B1F_MOUSE

0.15

0.38

0.40

n

Glyceraldehyde-3-phosphate dehydrogenase

G3P_MOUSE

0.12

0.37

0.32

c

Stress-70 protein

GRP75_MOUSE

0.02

0.09

0.26

m

Lysosomal alpha-glucosidase

LYAG_MOUSE

0.00

0.02

0.24

g

60 kDa heat shock protein

CH60_MOUSE

0.04

0.16

0.23

m

Probable ATP-dependent RNA helicase DDX17

DDX17_MOUSE

0.01

0.06

0.23

s

Glycogenin-1

GLYG_MOUSE

0.13

0.66

0.19

c

Probable ATP-dependent RNA helicase DDX5

DDX5_MOUSE

0.02

0.09

0.19

s

ATP synthase subunit beta

ATPB_MOUSE

0.06

0.31

0.18

m

Cleavage and polyadenylation specificity factor subunit 6

CPSF6_MOUSE

0.02

0.15

0.16

s

Histone H2A type 1-F †

H2A1F_MOUSE

0.10

0.62

0.16

n

Heterogeneous nuclear ribonucleoprotein H2

HNRH2_MOUSE

0.02

0.14

0.15

s

Pre-mRNA-splicing factor 38B

PR38B_MOUSE

0.01

0.10

0.14

s

Major vault protein

MVP_MOUSE

0.04

0.30

0.14

c

Heterogeneous nuclear ribonucleoprotein M

HNRPM_MOUSE

0.02

0.12

0.13

s

ATP synthase subunit alpha

ATPA_MOUSE

0.04

0.30

0.12

m

Glycogen synthase 1, muscle

Q8VEB0_MOUSE

0.45

4.36

0.10

g

Tubulin beta-2B chain †

TBB2B_MOUSE

0.01

0.14

0.10

c

ATP synthase subunit O

ATPO_MOUSE

0.02

0.20

0.10

m

Putative RNA-binding protein Luc7-like 2

LC7L2_MOUSE

0.04

0.35

0.10

s

Putative RNA-binding protein Luc7-like 1

LUC7L_MOUSE

0.02

0.18

0.10

s

Cleavage and polyadenylation specificity factor subunit 5

CPSF5_MOUSE

0.09

0.90

0.10

s

Trifunctional enzyme subunit alpha

ECHA_MOUSE

0.01

0.11

0.09

m

ATP-dependent RNA helicase DDX3X

DDX3X_MOUSE

0.02

0.20

0.08

s

Splicing factor, arginine/serine-rich 3

SFRS3_MOUSE

0.14

1.88

0.07

s

Splicing factor U2AF 65 kDa subunit

U2AF2_MOUSE

0.02

0.34

0.07

s

Cisplatin resistance-associated overexpressed protein

CROP_MOUSE

0.03

0.53

0.05

s

  1. The final purification of glycogen-associated proteins involved elution with malto-oligosaccharides, which competes with the glycogen molecule for specific interactions with the glycogen-associated proteins. To provide a measure of specific interaction with the glycogen molecule, proteins common to both the malto-oligosaccharide soluble and insoluble datasets were compared. Exponentially modified Protein Abundance Index (emPAI) values for each protein in the malto-oligosaccharide soluble (sol) and insoluble (insol) datasets were divided by the emPAI value for glycogen phosphorylase, a reference protein known to specifically interact with the glycogen molecule [10, 35]. These relative measures of binding to the glycogen particle were then directly compared: sol / insol = ((emPAI of protein in soluble dataset / emPAI of soluble glycogen phosphorylase) / (emPAI of protein in insoluble dataset / emPAI of insoluble glycogen phosphorylase)). This calculation provides a measure of relative binding to the glycogen molecule and allows comparison between differently sized datasets. Proteins are listed in order of relative solubility with malto-oligosaccharides (sol/insol). The reference protein, glycogen phosphorylase, is indicated in bold. Values greater than one represent proteins that are relatively more soluble than glycogen phosphorylase. The subcellular localization of proteins common to both soluble and insoluble datasets is also listed (compartment).
Back to article page