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Table 1 Differentially expressed proteins and their relative changes induced by heat stress in Vista leaves as determined by 2-DE

From: Heat stress-induced response of the proteomes of leaves from Salvia splendens Vista and King

Spot no. a

Protein

NCBI accession no. b

Reference organism

kDa/pI c

Score d

MASCOT peptide matches

SC (%) e

Average fold change (Mean ± SD) f

Biological process g

Cellular compartment h

Molecular function i

Upregulated

D01

Mthsc70-1

gi|297798080

Arabidopsis lyrata subsp. lyrata

73.37/5.52

169

14

20

2.42 ± 0.22

Protein folding

Unknown

ATP binding

D02

Mthsc70-1

gi|297798080

Arabidopsis lyrata subsp. lyrata

73.37/5.52

214

11

16

2.11 ± 0.13

Protein folding

Unknown

ATP binding

D04

Chloroplast ribulose-1,5-bisphosphate carboxylase activase

gi|225580059

Solenostemon scutellarioides

47.71/8.16

219

11

23

2.81 ± 0.34

Stress response

Chloroplast

ATP binding

D05

Chloroplast ribulose-1,5-bisphosphate carboxylase activase

gi|225580059

Solenostemon scutellarioides

47.71/8.16

109

9

21

2.60 ± 0.16

Stress response

Chloroplast

ATP binding

D06

Chloroplast ribulose-1,5-bisphosphate carboxylase activase

gi|225580059

Solenostemon scutellarioides

47.71/8.16

114

3

9

3.60 ± 0.47

Stress response

Chloroplast

ATP binding

D09

Chlorophyll a/b binding protein

gi|398599

Amaranthus hypochondriacus

28.71/5.68

98

3

17

1.89 ± 0.17

Photosynthesis

Chloroplast

Metal ion binding

D10

Cytochrome B6-F complex iron sulfur subunit 2

gi|146454656

Sonneratia caseolaris

16.83/5.75

81

3

32

1.97 ± 0.09

Electron transport

Chloroplast

Oxidoreductase

D11

Class-1 LMW heat shock protein

gi|25044839

Ananas comosus

17.56/6.77

182

3

16

7.47 ± 0.43

Stress response

Nucleus

Unknown

D12

Class-1 LMW heat shock protein

gi|25044839

Ananas comosus

17.56/6.77

211

4

21

9.16 ± 0.33

Stress response

Nucleus

Unknown

D13

Unknown

gi|224286712

Picea sitchensis

13.08/6.34

80

1

13

2.24 ± 0.12

Unknown

Unknown

Unknown

Downregulated

C02

Predicted protein

gi|224069527

Populus trichocarpa

90.78/5.05

131

9

16

−1.83 ± 0.11

Unknown

Unknown

Unknown

C03

Ribulose-1,5-bisphosphate carboxylase subunit

gi|46326312

Salvia coccinea

50.28/6.23

247

15

29

−1.74 ± 0.07

Photosynthesis

Chloroplast

Transferase

C04

Atsco1 /Cpef-g

gi|297837151

Arabidopsis lyrata subsp. lyrata

86.61/5.52

178

13

19

−1.53 ± 0.08

Unknown

Intracellular

GTP binding

C05

hypothetical protein

gi|225426927

Vitis vinifera

88.91/5.09

157

15

23

−1.79 ± 0.14

Unknown

Unknown

Unknown

C10

FtsH-like protein Pftf precursor

gi|4325041

Nicotiana tabacum

74.51/6.00

271

14

27

−1.70 ± 0.24

Protein catabolic process

Membrane

ATP binding

C12

Glucose-1-phosphate adenylyltransferase

gi|297835714

Arabidopsis lyrata subsp. lyrata

57.65/6.54

81

7

13

−1.57 ± 0.25

Starch biosynthesis

Unknown

ATP binding

C13

GDP-D-mannose-3′,5′-epimerase

gi|223469963

Ribes nigrum

42.94/5.88

207

10

34

−1.68 ± 0.09

Cellular metabolic process

Unknown

Catalytic activity

C15

Unknown

gi|217071972

Medicago truncatula

19.00/7.74

97

6

44

−1.94 ± 0.33

Unknown

Unknown

Unknown

C16

Putative actin protein

gi|300429861

Salvia miltiorrhiza

41.89/5.38

182

11

43

−1.74 ± 0.14

Transcription regulation

Nucleus

Protein binding

C17

NAD dependent epimerase/dehydratase, putative

gi|255542956

Ricinus communis

42.72/8.52

179

6

18

−2.06 ± 0.26

Cellular metabolic process

Unknown

Catalytic activity

C18

Degp1

gi|338858724

Arabidopsis halleri subsp. gemmifera

21.93/5.75

73

3

24

−1.53 ± 0.06

Stress response

Chloroplast

Serine-type endopeptidase activity

C19

Unnamed protein product

gi|296086699

Vitis vinifera

31.31/5.74

135

5

16

−1.53 ± 0.24

Unknown

Unknown

Unknown

C20

Ferredoxin--NADP reductase, putative

gi|255538962

Ricinus communis

40.74/8.7

113

7

17

−1.71 ± 0.16

Electron transport

Membrane

Protein binding

  1. aAs indicated in Figure 2A. bNCBI accession number. cTheoretical isoelectric points (pI values) and molecular masses (kDa). dMascot score. eSequence coverage. fSpot abundance is expressed as the ratio of the intensities of the heat-stressed to the corresponding control proteins. Positive values, upregulated; negative values, downregulated. g–iBiological processes, cellular compartments, and molecular functions were inferred from those reported in the UniProt database.