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Table 2 Differentially expressed proteins and their relative changes induced by heat stress in King leaves as determined by 2-DE

From: Heat stress-induced response of the proteomes of leaves from Salvia splendens Vista and King

Spot no. a Protein NCBI accession no. b Reference organism kDa/pI c Score d MASCOT peptide matches SC (%) e Average fold change (Mean ± SD) f Biological process g Cellular compartment h Molecular function i
Upregulated
B01 Hypothetical protein VITISV_009951 gi|147842424 Vitis vinifera 89.30/5.28 190 17 24 1.89 ± 0.19 Unknown Unknown Unknown
B03 Heat shock protein 83 gi|1708314 Ipomoea nil 81.06/4.95 296 20 27 1.62 ± 0.29 Stress response Cytoplasm Unfolded protein binding
B04 Heat shock protein 83 gi|1708314 Ipomoea nil 81.06/4.95 300 16 22 1.61 ± 0.14 Stress response Cytoplasm Unfolded protein binding
B05 ER-binding protein gi|57639078 Malus pumila 73.76/5.14 113 6 11 2.51 ± 0.37 Unknown Unknown ATP binding
B06 Unknown gi|148906951 Picea sitchensis 75.93/5.58 147 10 19 1.52 ± 0.25 Unknown Unknown Unknown
B07 Unknown gi|148907083 Picea sitchensis 75.84/5.46 168 9 17 1.54 ± 0.16 Unknown Unknown Unknown
B08 Transketolase, putative gi|255541252 Ricinus communis 81.62/6.52 88 8 12 2.61 ± 0.09 Energy reserve metabolic process Cytosol Unknown
B09 Precursor of protein cell division protease ftsh-like protein gi|224065699 Populus trichocarpa 75.74/6.0 238 19 29 6.31 ± 0.47 Cell division Membrane ATP binding
B10 Predicted protein gi|224098390 Populus trichocarpa 71.62/5.14 305 18 25 3.30 ± 0.35 Unknown Unknown Unknown
B11 Predicted protein gi|224098390 Populus trichocarpa 71.62/5.14 157 16 23 9.55 ± 1.07 Unknown Unknown Unknown
B12 Predicted protein gi|326499406 Hordeum vulgare subsp. vulgare 71.62/5.09 219 14 18 2.12 ± 0.43 Unknown Unknown Unknown
B13 Cytosolic heat shock 70 protein gi|2642648 Spinacia oleracea 71.84/5.09 82 12 22 9.23 ± 0.97 Stress response Cytoplasm ATP binding
B14 Chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase activase gi|225580059 Solenostemon scutellarioides 47.71/8.16 219 11 23 1.94 ± 0.12 Stress response Chloroplast ATP binding
B15 Chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase activase gi|225580059 Solenostemon scutellarioides 47.71/8.16 114 3 9 1.57 ± 0.28 Stress response Chloroplast ATP binding
B16 Predicted protein gi|224056853 Populus trichocarpa 38.65/7.56 148 3 13 2.26 ± 0.14 Unknown Unknown Unknown
B17 Unknown gi|118486395 Populus trichocarpa 18.46/5.08 85 3 18 9.61 ± 0.55 Unknown Unknown Unknown
B19 Class-1 low-molecular-weight heat shock protein gi|25044839 Ananas comosus 17.56/6.77 182 3 16 7.91 ± 0.42 Stress response Unknown Unknown
B20 Class-1 low-molecular-weight heat shock protein gi|25044839 Ananas comosus 17.56/6.77 211 4 21 6.86 ± 0.49 Stress response Unknown Unknown
B21 Unknown gi|224286712 Picea sitchensis 13.08/6.34 80 1 13 1.95 ± 0.13 Unknown Unknown Unknown
B22 Unknown gi|224286712 Picea sitchensis 13.08/6.34 95 1 13 1.99 ± 0.23 Unknown Unknown Unknown
Downregulated
A01 RuBisCO large subunit-binding protein subunit alpha, chloroplastic gi|1710807 Pisum sativum 62.00/5.15 103 10 19 −1.87 ± 0.17 Photosynthesis Chloroplast stroma ATP binding
A02 ATP synthase beta subunit gi|123325679 Luvunga sp. TT 364 50.00/5.1 77 8 26 −1.89 ± 0.26 ADP biosynthetic process Mitochondrial ATP binding
A03 ATP synthase CF1 beta subunit gi|306485983 Franklinia alatamaha 51.00/5.35 417 19 59 −1.66 ± 0.11 ADP biosynthetic process Chloroplast ATP binding
A05 Unknown gi|217071972 Medicago truncatula 19.00/7.74 97 6 44 −2.28 ± 0.28 Unknown Unknown Unknown
A06 Similar to Probable pyridoxal biosynthesis protein PDX1 gi|225434584 Vitis vinifera 33.33/5.94 263 12 28 −1.71 ± 0.08 Pyridoxal phosphate biosynthetic process Unknown Catalytic activity
A09 Hypothetical protein LOC100382672 gi|293333399 Zea mays 32.02/9.24 91 4 13 −1.55 ± 0.33 Unknown Unknown Unknown
A10 Hypothetical protein gi|225437581 Vitis vinifera 31.73/7.71 105 4 19 −2.22 ± 0.21 Unknown Unknown Unknown
A11 Hypothetical protein gi|110742537 Arabidopsis thaliana 23.17/4.63 74 2 10 −1.85 ± 0.12 Unknown Unknown Unknown
  1. aAs indicated in Figure 2A. bNCBI accession number. cTheoretical isoelectric points (pI values) and molecular masses (kDa). dMascot score. eSequence coverage. fSpot abundance is expressed as the ratio of the intensities of the heat-stressed to the corresponding control proteins. Positive values, upregulated; negative values, downregulated. g–iBiological processes, cellular compartments, and molecular functions were inferred from those reported in the UniProt database.