Skip to main content

Table 2 Differentially expressed proteins and their relative changes induced by heat stress in King leaves as determined by 2-DE

From: Heat stress-induced response of the proteomes of leaves from Salvia splendens Vista and King

Spot no. a

Protein

NCBI accession no. b

Reference organism

kDa/pI c

Score d

MASCOT peptide matches

SC (%) e

Average fold change (Mean ± SD) f

Biological process g

Cellular compartment h

Molecular function i

Upregulated

B01

Hypothetical protein VITISV_009951

gi|147842424

Vitis vinifera

89.30/5.28

190

17

24

1.89 ± 0.19

Unknown

Unknown

Unknown

B03

Heat shock protein 83

gi|1708314

Ipomoea nil

81.06/4.95

296

20

27

1.62 ± 0.29

Stress response

Cytoplasm

Unfolded protein binding

B04

Heat shock protein 83

gi|1708314

Ipomoea nil

81.06/4.95

300

16

22

1.61 ± 0.14

Stress response

Cytoplasm

Unfolded protein binding

B05

ER-binding protein

gi|57639078

Malus pumila

73.76/5.14

113

6

11

2.51 ± 0.37

Unknown

Unknown

ATP binding

B06

Unknown

gi|148906951

Picea sitchensis

75.93/5.58

147

10

19

1.52 ± 0.25

Unknown

Unknown

Unknown

B07

Unknown

gi|148907083

Picea sitchensis

75.84/5.46

168

9

17

1.54 ± 0.16

Unknown

Unknown

Unknown

B08

Transketolase, putative

gi|255541252

Ricinus communis

81.62/6.52

88

8

12

2.61 ± 0.09

Energy reserve metabolic process

Cytosol

Unknown

B09

Precursor of protein cell division protease ftsh-like protein

gi|224065699

Populus trichocarpa

75.74/6.0

238

19

29

6.31 ± 0.47

Cell division

Membrane

ATP binding

B10

Predicted protein

gi|224098390

Populus trichocarpa

71.62/5.14

305

18

25

3.30 ± 0.35

Unknown

Unknown

Unknown

B11

Predicted protein

gi|224098390

Populus trichocarpa

71.62/5.14

157

16

23

9.55 ± 1.07

Unknown

Unknown

Unknown

B12

Predicted protein

gi|326499406

Hordeum vulgare subsp. vulgare

71.62/5.09

219

14

18

2.12 ± 0.43

Unknown

Unknown

Unknown

B13

Cytosolic heat shock 70 protein

gi|2642648

Spinacia oleracea

71.84/5.09

82

12

22

9.23 ± 0.97

Stress response

Cytoplasm

ATP binding

B14

Chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase activase

gi|225580059

Solenostemon scutellarioides

47.71/8.16

219

11

23

1.94 ± 0.12

Stress response

Chloroplast

ATP binding

B15

Chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase activase

gi|225580059

Solenostemon scutellarioides

47.71/8.16

114

3

9

1.57 ± 0.28

Stress response

Chloroplast

ATP binding

B16

Predicted protein

gi|224056853

Populus trichocarpa

38.65/7.56

148

3

13

2.26 ± 0.14

Unknown

Unknown

Unknown

B17

Unknown

gi|118486395

Populus trichocarpa

18.46/5.08

85

3

18

9.61 ± 0.55

Unknown

Unknown

Unknown

B19

Class-1 low-molecular-weight heat shock protein

gi|25044839

Ananas comosus

17.56/6.77

182

3

16

7.91 ± 0.42

Stress response

Unknown

Unknown

B20

Class-1 low-molecular-weight heat shock protein

gi|25044839

Ananas comosus

17.56/6.77

211

4

21

6.86 ± 0.49

Stress response

Unknown

Unknown

B21

Unknown

gi|224286712

Picea sitchensis

13.08/6.34

80

1

13

1.95 ± 0.13

Unknown

Unknown

Unknown

B22

Unknown

gi|224286712

Picea sitchensis

13.08/6.34

95

1

13

1.99 ± 0.23

Unknown

Unknown

Unknown

Downregulated

A01

RuBisCO large subunit-binding protein subunit alpha, chloroplastic

gi|1710807

Pisum sativum

62.00/5.15

103

10

19

−1.87 ± 0.17

Photosynthesis

Chloroplast stroma

ATP binding

A02

ATP synthase beta subunit

gi|123325679

Luvunga sp. TT 364

50.00/5.1

77

8

26

−1.89 ± 0.26

ADP biosynthetic process

Mitochondrial

ATP binding

A03

ATP synthase CF1 beta subunit

gi|306485983

Franklinia alatamaha

51.00/5.35

417

19

59

−1.66 ± 0.11

ADP biosynthetic process

Chloroplast

ATP binding

A05

Unknown

gi|217071972

Medicago truncatula

19.00/7.74

97

6

44

−2.28 ± 0.28

Unknown

Unknown

Unknown

A06

Similar to Probable pyridoxal biosynthesis protein PDX1

gi|225434584

Vitis vinifera

33.33/5.94

263

12

28

−1.71 ± 0.08

Pyridoxal phosphate biosynthetic process

Unknown

Catalytic activity

A09

Hypothetical protein LOC100382672

gi|293333399

Zea mays

32.02/9.24

91

4

13

−1.55 ± 0.33

Unknown

Unknown

Unknown

A10

Hypothetical protein

gi|225437581

Vitis vinifera

31.73/7.71

105

4

19

−2.22 ± 0.21

Unknown

Unknown

Unknown

A11

Hypothetical protein

gi|110742537

Arabidopsis thaliana

23.17/4.63

74

2

10

−1.85 ± 0.12

Unknown

Unknown

Unknown

  1. aAs indicated in Figure 2A. bNCBI accession number. cTheoretical isoelectric points (pI values) and molecular masses (kDa). dMascot score. eSequence coverage. fSpot abundance is expressed as the ratio of the intensities of the heat-stressed to the corresponding control proteins. Positive values, upregulated; negative values, downregulated. g–iBiological processes, cellular compartments, and molecular functions were inferred from those reported in the UniProt database.