Microscopy images of cultured rice protoplasts (from suspension cells) following the cell wall regeneration time course. CLSM was used to observe the protoplasts stained by a fluorescent dye, Fluorescent Brightener 28, with polysaccharide specific binding activities. The excitation wavelength at 492 nm and emission at 520 nm were used. The protoplast culture times are 0hrs (A), 4 hrs (B), and 48 hrs (C). The arrows point at the positions of cell wall syntheses. The magnification is revealed by the scale bar.