Microscopy images of isolated rice ( O. sativa) suspension cell and protoplast nuclei and Western blot analysis of purified nuclear proteins. (A) Image of purified suspension cell nuclei after DAPI staining. (B) Image of protoplast with cell wall regeneration (4 hrs) nuclei after DAPI staining. A small volume of the purified nuclei was stained with DAPI (0.5 μg/ml) for 5 minutes and images were taken under a DAPI-filter. The magnification is revealed by the scale bar. (C) Nuclei enrichment revealed by Western blots. Antibodies against H4, V-ATPase, E, and cFBPase were used to assess the protein quantity in the total protein fraction and suspension cell and protoplast nuclei, respectively. 20 μg of proteins were loaded in each lane.