Proteomic profiling for the identification of serum diagnostic biomarkers for abdominal and thoracic aortic aneurysms

Satoh, Kazumi; Maniwa, Tomoko; Oda, Teiji; Matsumoto, Ken-ichi

doi:10.1186/1477-5956-11-27

Proteome Science

Table 2 Proteins with differential levels in postsurgical sera compared with those in presurgical sera of TAA patients

From: Proteomic profiling for the identification of serum diagnostic biomarkers for abdominal and thoracic aortic aneurysms

Unused protScore ^a	% Coverage ^b	Peptides ^c(95%)	Uniplot number	Gene symbol	Protein name	iTRAQ ratio ^daverage ± SE	p value ^e	Molecular function
						(postsurgical vs.presurgical)	(patient vs. control)
*Increased proteins*
46.2	82.0	48	P02735	SAA1	Serum amyloid A protein	15.65 ± 3.40	-^f	Acute phase protein
16.0	34.8	9	P02741	CRP	C-reactive protein	4.31 ± 0.54	-^f	Acute phase protein
60.0	73.6	68	P02763	ORM1	Alpha-1-acid glycoprotein 1	2.61 ± 0.18	0.0002	Transporter
62.0	68.3	47	P01011	SERPINA3	Alpha-1-antichymotrypsin	2.39 ± 0.21	0.0010	Protease inhibitor
29.8	59.1	24	P02750	LRG1	Leucine-rich alpha-2-glycoprotein	2.11 ± 0.30	0.0235	Unknown
123.8	80.6	151	P01009	SERPINA1	Alpha-1-antitrypsin	1.50 ± 0.15	0.0397	Protease inhibitor
28.3	32.7	16	P02748	C9	Complement component C9	1.43 ± 0.15	0.0469	Complement
16.5	21.7	8	P09871	C1S	Complement C1s subcomponent	1.30 ± 0.07	-^f	Complement
*Decreased proteins*
30.2	38.0	15	P06396	GSN	Gelsolin	0.49 ± 0.05	0.0002	Actin scavenger
38.9	54.5	30	P02765	AHSG	Alpha-2-HS-glycoprotein	0.51 ± 0.09	0.0008	Extracellular matrix
19.3	89.0	14	P02652	APOA2	Apolipoprotein A-II	0.52 ± 0.09	0.0258	Transporter
14.2	27.2	7	P29622	SERPINA4	Kallistatin	0.56 ± 0.08	0.0085	Protease inhibitor
95.4	92.1	97	P02647	APOA1	Apolipoprotein A-I	0.59 ± 0.09	0.0473	Transporter
16.5	53.7	10	P02753	RBP4	Retinol-binding protein 4	0.60 ± 0.10	0.0027	Transfer/carrier protein
183.4	83.7	171	P02787	TF	Serotransferrin	0.60 ± 0.08	0.0021	Transfer/carrier protein
23.1	67.6	11	O95445	APOM	Apolipoprotein M	0.61 ± 0.05	0.0050	Transporter
36.3	46.3	19	P04196	HRG	Histidine-rich glycoprotein	0.61 ± 0.07	0.0061	Adapter protein
156.2	49.9	98	P02751	FN1	Fibronectin	0.65 ± 0.07	0.0261	Extracellular matrix
13.3	27.3	7	P27169	PON1	Serum paraoxonase/arylesterase 1	0.66 ± 0.07	0.0011	Hydrolase
16.4	22.3	7	P03952	KLKB1	Plasma kallikrein	0.66 ± 0.09	0.0191	Protease
12.0	78.2	7	P02655	APOC2	Apolipoprotein C-II	0.66 ± 0.14	0.0387	Transporter
289.1	78.8	228	P01023	A2M	Alpha-2-macroglobulin	0.66 ± 0.05	0.0027	Signaling molecule
59.1	41.9	34	P19823	ITIH2	Inter-alpha-trypsin inhibitor heavy chain H2	0.68 ± 0.08	0.0043	Protease inhibitor
28.4	38.4	14	P43652	AFM	Afamin	0.70 ± 0.08	0.0041	Transfer/carrier protein
30.5	44.7	18	P05546	SERPIND1	Heparin cofactor 2	0.76 ± 0.09	0.0424	Protease inhibitor

^aA score of protein confidence(ProtScore) for a detected protein that is calculated from the peptide confidence from spectra that are not already “used” by higher scoring proteins in the experiments.
^bThe percentage of matching amino acids from identified peptides.
^cThe number of distinct peptides with at least 95% confidence in the experiments.
^diTRAQ ratio of postsurgical sera compared with those in presurgical sera (postsurgical vs. presurgical).
^eStatistical analysis of iTRAQ ratio of postsurgery/presurgery of patients’ sera compared with that of T2/T1 of control volunteers’ sera (patient vs. control).
^fProtein that did not appear in control sera.

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ISSN: 1477-5956

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