Comparative secretome analysis of four isogenic Bacillus clausii probiotic strains

Lippolis, Rosa; Siciliano, Rosa Anna; Mazzeo, Maria Fiorella; Abbrescia, Anna; Gnoni, Antonio; Sardanelli, Anna Maria; Papa, Sergio

doi:10.1186/1477-5956-11-28

Proteome Science

Table 1 Secreted proteins differentially expressed by the four probiotic strains of B. clausii , as identified by PMF strategy

From: Comparative secretome analysis of four isogenic Bacillus clausii probiotic strains

Functional classification	Match ID ^a	Gene ^b	Protein name	Accession Number (NCBInr)	Kegg Entry ^c(organism Bcl)	M.W. ^d(kDa)	pI ^e	Mascot Score	Number of matched peptides	Sequence Coverage(%)	Localization	References
Carbohydrate metabolism	309	eno	Phosphopyruvate hydratase (Enolase)	gi\|56964781	ABC3017	46226	4.66	294	19	58	Cytoplasm. Secreted. Cell surface without export signal	Antelmann et al. Genome Res. 2001, 11:1484
	382	adhA	Alcohol dehydrogenase	gi\|56961828	ABC0046	37979	5.26	230	15	69	Cytoplasm.
	107	lpd	Dihydrolipoamide dehydrogenase	gi\|56964182	ABC2452	49579	5.25	261	21	47	Membrane. Extracellular Membrane bound	Engels et al. BBA. 1997, 1340:33
	207	lpd	Dihydrolipoamide dehydrogenase	gi\|56964182	ABC2452	49579	5.19	207	18	67	Membrane. Extracellular Membrane bound	Engels et al. BBA. 1997, 1340:33
			Succinate-semialdehyde dehydrogenase	gi\|56962111	ABC0331	51161	5.23	109	15	44
	216	acsA	Acetyl-CoA synthetase	gi\|56964524	ABC2760	64388	5.24	293	26	50
	439	mdh	Malate dehydrogenase	gi\|56964478	ABC2713	33576	5.16	119	10	62	Cytoplasm Secreted without export signal	Antelmann et al Genome Res. 2001, 11:1484
			Sugar Phosphate isomerase/epimerase	gi\|56962569	ABC0795	36078	5.18	227	15	83
	231	pgi	Glucose-6-phosphate isomerase	gi\|56964670	ABC2906	50356	5.25	378	28	87
Peptides/Nickel transport system	33		Oligopeptide ABC transporter substrate-binding protein	gi\|56964179	ABC2414	65042	4.07	271	24)	55	Secreted with export signal	Antelmann et al. Genome Res. 2001, 11:1484
Peptides/Nickel transport system			Oligopeptide ABC transporter substrate-binding protein	gi\|56964854	ABC3090	68534	4.09	257	25	50	Secreted with export signal	Antelmann et al. Genome Res. 2001, 11:1484
Environmental information Processing Membrane transport	247	mntA	Mn2+/Zn2+ ABC transporter substrate-binding protein	gi\|56965719	ABC3961	34305	3.78	65	4	27	Secreted with export signal	Antelmann et al. Genome Res. 2001, 11:1484
Proteases/Peptidases	532	aprE	Chain A, Alkaline M-Protease Form I Crystal Structure	gi\|56966974	ABC0761	26707	9.30	110	8	40	Secreted without export signal	Antelmann et al. Genome Res. 2001, 11:1484
	593	aprE	Chain A, Alkaline M-Protease Form I Crystal Structure	gi\|56966974	ABC0761	26707	9.30	147	8	40	Secreted. without export signal	Antelmann et al. Genome Res. 2001, 11:1484
	693	aprE	Chain A, Alkaline M-Protease Form I Crystal Structure	gi\|56966974	ABC0761	26707	9.30	97	5/6	27	Secreted without export signal	Antelmann et al. Genome Res. 2001, 11:1484
	245	ampS	Aminopeptidase	gi\|56963895	ABC2130	45195	5.06	197	18	46	Secreted with export signal	Wei Wang et. al. Proteome Science. 2006, 4:19
Antioxidant defence	120	cat	Catalase	gi\|56963055	ABC1283	55939	5.52	360	31	82	Secreted without export signal	Antelmann et al. Genome Res. 2001, 11:1484
	156	cat	Catalase	gi\|56963055	ABC1283	55939	5.52	375	28	67	Secreted without export signal	Antelmann et al Genome Res. 2001, 11:1484
	164	cat	Catalase	gi\|56963055	ABC1283	55939	5.52	295	20	56	Secreted without export signal	Antelmann et al. Genome Res. 2001, 11:1484
	694		2-cys Peroxiredoxin	gi\|56964193	ABC2428	20402	4.56	143	9		Secreted	Min-Ho Cho et al. Molecular Biochemical Parasitology, 2005, 143:80
	602	sodA	Manganese Superoxide dismutase	gi\|56963480	ABC3961	22330	5.41	132	7	59	Secreted without signal peptides	Antelmann et al. Genome Res. 2001, 11:1484
Energy metabolism	485		Carbonic anhydrase	gi\|56964946	ABC3182	29268	4.37	98	6	22	Secreted	Oviya M et al. Prep Biochem Biotechnol. 2012 42:462
RNA synthesis	281		Transcriptional regulator	gi\|56962515	ABC0741	47467	5.35	341	26	77	Secreted with signal peptides	H Tjalsma et al. Microbiology and Molecular Biology reviews, 2004, 68:207
Structural protein	451	hag	Flagellin protein	gi\|56965461	ABC3699	32042	4.52	121	11	34	Cytoplasm. Secreted without export signal	Antelmann et al. Genome Res. 2001, 11:1484
Cell-Wall metabolism	528	cwlC	Acetylmuramoyl-L-Alanine amidase	gi\|56962701	ABC0927	27617	8.89	165	12	54	Cell surface	Nouwens et al. Microbiology 2003, 149: 1311
Environmental Information Processing	528	resD	Two-component response regulator	gi\|56963600	ABC1835	27512	5.17	95	9	40
Molecular Chaperones	114	groEL	molecular chaperone GroEL	gi\|56962656	ABC0882	57240	4.76	33		66	Secreted	Beck et al. FEMS Microbiol Lett 2009, 297:6166
	639		Hypothetical protein ABC0920	gi/56962694		14960	4.40	109	6	73	Secreted	Antelmann et al. Genome Res. 2001 11: 1484
	673		Hypothetical protein ABC2092	gi\|56963857	ABC2092	13501	5.41	130	10	84	Secreted	Antelmann et al. Genome Res. 2001 11: 1484
	659		Hypothetical protein ABC2108	gi\|56963873	ABC2108	13099	5.55	120	8	59	Secreted	Antelmann et al. Genome Res. 2001 11: 1484

a. ID Spot numbers refer to Figure 4.
b. Gene.
c. Kegg Entry. Organism: B. clausii KSM-K16 (Bcl).
d. Theoretical molecular weight (kDa).
e. Theoretical isoelectric point.

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ISSN: 1477-5956

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