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Table 1 Secreted proteins differentially expressed by the four probiotic strains of B. clausii , as identified by PMF strategy

From: Comparative secretome analysis of four isogenic Bacillus clausii probiotic strains

Functional classification Match ID a Gene b Protein name Accession Number (NCBInr) Kegg Entry c(organism Bcl) M.W. d(kDa) pI e Mascot Score Number of matched peptides Sequence Coverage(%) Localization References
Carbohydrate metabolism 309 eno Phosphopyruvate hydratase (Enolase) gi|56964781 ABC3017 46226 4.66 294 19 58 Cytoplasm. Secreted. Cell surface without export signal Antelmann et al. Genome Res. 2001, 11:1484
382 adhA Alcohol dehydrogenase gi|56961828 ABC0046 37979 5.26 230 15 69 Cytoplasm.  
107 lpd Dihydrolipoamide dehydrogenase gi|56964182 ABC2452 49579 5.25 261 21 47 Membrane. Extracellular Membrane bound Engels et al. BBA. 1997, 1340:33
207 lpd Dihydrolipoamide dehydrogenase gi|56964182 ABC2452 49579 5.19 207 18 67 Membrane. Extracellular Membrane bound Engels et al. BBA. 1997, 1340:33
   Succinate-semialdehyde dehydrogenase gi|56962111 ABC0331 51161 5.23 109 15 44   
216 acsA Acetyl-CoA synthetase gi|56964524 ABC2760 64388 5.24 293 26 50   
439 mdh Malate dehydrogenase gi|56964478 ABC2713 33576 5.16 119 10 62 Cytoplasm Secreted without export signal Antelmann et al Genome Res. 2001, 11:1484
   Sugar Phosphate isomerase/epimerase gi|56962569 ABC0795 36078 5.18 227 15 83   
231 pgi Glucose-6-phosphate isomerase gi|56964670 ABC2906 50356 5.25 378 28 87   
Peptides/Nickel transport system 33   Oligopeptide ABC transporter substrate-binding protein gi|56964179 ABC2414 65042 4.07 271 24) 55 Secreted with export signal Antelmann et al. Genome Res. 2001, 11:1484
   Oligopeptide ABC transporter substrate-binding protein gi|56964854 ABC3090 68534 4.09 257 25 50 Secreted with export signal Antelmann et al. Genome Res. 2001, 11:1484
Environmental information Processing Membrane transport 247 mntA Mn2+/Zn2+ ABC transporter substrate-binding protein gi|56965719 ABC3961 34305 3.78 65 4 27 Secreted with export signal Antelmann et al. Genome Res. 2001, 11:1484
Proteases/Peptidases 532 aprE Chain A, Alkaline M-Protease Form I Crystal Structure gi|56966974 ABC0761 26707 9.30 110 8 40 Secreted without export signal Antelmann et al. Genome Res. 2001, 11:1484
593 aprE Chain A, Alkaline M-Protease Form I Crystal Structure gi|56966974 ABC0761 26707 9.30 147 8 40 Secreted. without export signal Antelmann et al. Genome Res. 2001, 11:1484
693 aprE Chain A, Alkaline M-Protease Form I Crystal Structure gi|56966974 ABC0761 26707 9.30 97 5/6 27 Secreted without export signal Antelmann et al. Genome Res. 2001, 11:1484
245 ampS Aminopeptidase gi|56963895 ABC2130 45195 5.06 197 18 46 Secreted with export signal Wei Wang et. al. Proteome Science. 2006, 4:19
Antioxidant defence 120 cat Catalase gi|56963055 ABC1283 55939 5.52 360 31 82 Secreted without export signal Antelmann et al. Genome Res. 2001, 11:1484
156 cat Catalase gi|56963055 ABC1283 55939 5.52 375 28 67 Secreted without export signal Antelmann et al Genome Res. 2001, 11:1484
164 cat Catalase gi|56963055 ABC1283 55939 5.52 295 20 56 Secreted without export signal Antelmann et al. Genome Res. 2001, 11:1484
694   2-cys Peroxiredoxin gi|56964193 ABC2428 20402 4.56 143 9   Secreted Min-Ho Cho et al. Molecular Biochemical Parasitology, 2005, 143:80
602 sodA Manganese Superoxide dismutase gi|56963480 ABC3961 22330 5.41 132 7 59 Secreted without signal peptides Antelmann et al. Genome Res. 2001, 11:1484
Energy metabolism 485   Carbonic anhydrase gi|56964946 ABC3182 29268 4.37 98 6 22 Secreted Oviya M et al. Prep Biochem Biotechnol. 2012 42:462
RNA synthesis 281   Transcriptional regulator gi|56962515 ABC0741 47467 5.35 341 26 77 Secreted with signal peptides H Tjalsma et al. Microbiology and Molecular Biology reviews, 2004, 68:207
Structural protein 451 hag Flagellin protein gi|56965461 ABC3699 32042 4.52 121 11 34 Cytoplasm. Secreted without export signal Antelmann et al. Genome Res. 2001, 11:1484
Cell-Wall metabolism 528 cwlC Acetylmuramoyl-L-Alanine amidase gi|56962701 ABC0927 27617 8.89 165 12 54 Cell surface Nouwens et al. Microbiology 2003, 149: 1311
Environmental Information Processing 528 resD Two-component response regulator gi|56963600 ABC1835 27512 5.17 95 9 40   
Molecular Chaperones 114 groEL molecular chaperone GroEL gi|56962656 ABC0882 57240 4.76 33   66 Secreted Beck et al. FEMS Microbiol Lett 2009, 297:6166
  639   Hypothetical protein ABC0920 gi/56962694   14960 4.40 109 6 73 Secreted Antelmann et al. Genome Res. 2001 11: 1484
  673   Hypothetical protein ABC2092 gi|56963857 ABC2092 13501 5.41 130 10 84 Secreted Antelmann et al. Genome Res. 2001 11: 1484
  659   Hypothetical protein ABC2108 gi|56963873 ABC2108 13099 5.55 120 8 59 Secreted Antelmann et al. Genome Res. 2001 11: 1484
  1. a. ID Spot numbers refer to Figure 4.
  2. b. Gene.
  3. c. Kegg Entry. Organism: B. clausii KSM-K16 (Bcl).
  4. d. Theoretical molecular weight (kDa).
  5. e. Theoretical isoelectric point.