Skip to main content
Figure 2 | Proteome Science

Figure 2

From: A new type of protein chip to detect hepatocellular carcinoma-related autoimmune antibodies in the sera of hepatitis C virus-positive patients

Figure 2

The first-designed HCV-related HCC patient autoantigen protein chip. (A) The five recombinant proteins confirmed by SDS-PAGE. The proteins were 6×His-GFP-5×Cys (G), 6×His-GFP-HSP70-C-terminal domain-5×Cys (HC), 6×His-GFP-HSP70-N-terminal domain-5×Cys (HN), 6× His-GFP-SOD2-5×Cys (S), and 6×His-GFP-PRDX6-5×Cys (P). (B-G) The first-designed protein chip and its certification. (B) GFP fluorescence from the protein chip as detected by fluorescence microscopy. The proteins (128 fmol per spot) were covalently cross-linked to maleimide-incorporated diamond-like carbon chip. Spots were 200 μm in diameter. Abbreviations for proteins are same as in A. (C-G) Full color fluorescence images of immobilized protein by ProscanArray. The protein chips were detected after denaturation and incubation of primary anti-His antibody (C), anti-GFP (D), anti-HSP70 (E), anti-SOD2 (F), and anti-PRDX6 (G), visualized using fluorescent secondary antibodies.

Back to article page

Proteome Science

ISSN: 1477-5956