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Figure 3 | Proteome Science

Figure 3

From: A new type of protein chip to detect hepatocellular carcinoma-related autoimmune antibodies in the sera of hepatitis C virus-positive patients

Figure 3

Effect of pre-treatment for autoantibody detection on chip. Shown are GFP fluorescence images from a non-pre-treated chip (upper row) and a SDS/2ME/heat pre-treated chip (lower row) incubated with serum no. 150. Shown are fluorescence microscopic images before (prior to step 1 in Figure 1) and after pre-treatment (after step 2) and following incubation with serum (step 10). The Alexa555 images taken at step 10 show the total amount of protein bound to the plate, and the Alexa633 images show the amount of serum autoantibodies present against the C-terminus of HSP70 (HC). Pre-treatment with SDS/2ME improved the Alexa555 signal and reduced the Alexa633 background. Abbreviations for proteins are same as Figure  2.

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