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Figure 1 | Proteome Science

Figure 1

From: Comparison of first dimension IPG and NEPHGE techniques in two-dimensional gel electrophoresis experiment with cytosolic unfolded protein response in Saccharomyces cerevisiae

Figure 1

2DE of yeast whole cell lysates using IPG (A-C) and NEPHGE (D-F) based methods at standard protein load. The same samples from control cells ( transformed with empty vector pFGG3; A, D) and MeH ( pFGG3-MeH transformant; B, E) or MeN ( pFGG3-MeN; C, F) expressing cells were loaded onto IPG strips (50 μg of total protein in each strip) and NEPHGE gels (30 μg of total protein in each gel). Approximate pI values are indicated below the gels (pH 3–10 gradient was used in both methods). Dashed line indicates approximate zone of neutral pI 7.0, which separates acidic (on the left, pI < 7) and basic (on the right, pI > 7) protein spots. Protein molecular weight markers (M) are loaded onto IPG-based 2D gels, their masses are indicated at the right (kDa). Arrows point to the spots described in Table 3. Solid arrows indicate protein spots that were identified in our previous work [17], whereas dotted arrows point to additional spots identified by MS in this study. Quantitative analysis of each indicated protein spot is presented in Table 3.

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Proteome Science

ISSN: 1477-5956