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Figure 2 | Proteome Science

Figure 2

From: Comparison of first dimension IPG and NEPHGE techniques in two-dimensional gel electrophoresis experiment with cytosolic unfolded protein response in Saccharomyces cerevisiae

Figure 2

2DE of yeast whole cell lysates using IPG (A-C) and NEPHGE (D-F) based methods at high protein load. The same concentrated samples from control cells ( transformed with empty vector pFGG3; A, D) and MeH ( pFGG3-MeH transformant; B, E) or MeN ( pFGG3-MeN; C, F) expressing cells were loaded onto IPG strips (100 μg of total protein in each strip) and NEPHGE gels (100 μg of total protein in each gel). Original scan of one of the replicas is shown for comparison (six gels were being scanned in parallel at the same time). The references are the same as in Figure 1.

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Proteome Science

ISSN: 1477-5956