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Figure 3 | Proteome Science

Figure 3

From: Comparison of first dimension IPG and NEPHGE techniques in two-dimensional gel electrophoresis experiment with cytosolic unfolded protein response in Saccharomyces cerevisiae

Figure 3

Verification of proteomic results by immunoblot. SDS-PAGE (A) and Western blot (B) analysis of crude yeast lysates are shown. Lysates were prepared from galactose-induced yeast cells of S. cerevisiae AH22 strain transformed with empty vector (control, lane C) or plasmids expressing MeH (lane H) or MeN (lane N). (A) Coomassie blue-stained gel. Solid arrows indicate bands of recombinant MeH (lane H) and MeN (lane N) proteins. Lane M - prestained protein ladder with molecular weights of bands indicated at the left. (B) Western blot analysis using the same samples transferred onto nitrocellulose membrane. The blots were probed with antibodies against yeast Kar2 and Sis1 proteins. GAPDH was used as loading control.

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