Subcellular localization and COG classification of identified proteins. Identified proteins were classified according to their subcellular localization (A) and Cluster of Orthologous Group (B). In each diagram the first dark column represents the total number of proteins identified in the group. Grey and white columns are wt and mutant values of pellets in either GP (first column) or SP (second column) respectively. Hatched columns are proteins identified in SNs. In diagram A, c = cytoplasmic, cm = cytoplasmic membrane, e = extracellular, om = outer membrane, p = periplasmic and u = unknown localization. In diagram B, J = translation, ribosomal structure and biogenesis, K = transcription, L = DNA replication, recombination and repair, D = cell division and chromosome partitioning, O = posttranslational modification, protein turnover, chaperones, M = cell envelope biogenesis, outer membrane, N = cell motility and secretion, P = inorganic ion transport and metabolism, T = signal transduction mechanisms, C = energy production and conversion, G = carbohydrate transport and metabolism, E = amino acid transport and metabolism, F = nucleotide transport and metabolism, H = coenzyme metabolisme, I = lipid metabolism, Q = secondary metabolites biosynthesis, transport and catabolism, R = poorly characterized with general function prediction, S = poorly characterized with unknown function, and U = unclassified group.