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Table 2 Differentially expressed proteins in P32R48 maize hybrid from control (non-genetically modified) and genetically modified P32R48YG (MON810) maize hybrid grown under two different agroecosystems (Campos Novos and Chapecó) in Brazil

From: Comparative proteomic analysis of genetically modified maize grown under different agroecosystems conditions in Brazil

Spot

Fold variation a

Mascot score

Protein name b

Matched pep

NCBI Accession

Theor. MW

Theor. PI

Exp. MW

Exp. PI

Celullar component

Function c

Category

Campos Novos

492

Non-GM

1337

Adp-glucose pyrophosphorylase small subunit [zea mays]

14

gi|14582768

56

6.48

50

5.47

Amiloplast, apoplast, chloroplast, cytosol

Starch biosyntesis (nucleotydiltransferase)

Carbohidrate metabolism

629

Non-GM

848

Fructose-bisphosphate aldolase [zea mays]*

10

gi|223975775

38

6.37

38

5.65

Cell wall, chloroplast, cytosol, mitochondrion, nucleolus, membrane

Glycolysis/gluconeogenesis

Carbohidrate metabolism

507

GM

714

Gadph (383 aa) [zea mays]

8

gi|22240

41

7.21

39

6.18

Chloroplast

Glycolysis/gluconeogenesis

Carbohidrate metabolism

638

GM

788

Lactoylglutathione lyase [zea mays]*

15

gi|194701526

37

5.87

31

5.29

Chloroplast, peroxisome, plasma membrane, vacuole

Pyruvate metabolism

Carbohidrate metabolism

741

Non-GM

595

Lactoylglutathione lyase [zea mays]*

9

gi|194701526

37

5.87

31

5.54

Chloroplast, peroxisome, plasma membrane, vacuole

Pyruvate metabolism

Carbohidrate metabolism

589

Non-GM

781

Sedoheptulose bisphosphatase1 [zea mays]

11

gi|226506366

42

6.08

41

6.16

Cytosol

Calvin cycle

Carbohidrate metabolism

602

GM

797

Ferredoxin--nadp reductase, leaf isozyme [zea mays]

9

gi|226497434

41

8.53

33

5.84

Apoplast, chloroplast

Photophosphorylation

Energy metabolism

983

Non-GM

365

Thylakoid lumenal 19 kda protein [zea mays]

6

gi|226491484

27

5.48

19

4.19

Chloroplast

Photophosphorylation

Energy metabolism

794

GM

590

Adenine phosphoribosyltransferase [hordeum vulgare subsp. Vulgare]*

8

gi|194701624

19

5.14

22

5.08

Cytoplasm

Adenine metabolism (glycosyltransferase)

Genetic information processing

930

GM

335

Atp-dependent clp protease atp-binding subunit clpa [zea mays]

3

gi|226507418

25

9.39

16

6.47

Chloroplast

Proteolysis (nucleotide binding)

Genetic information processing

984

GM

449

Uncharacterized protein loc100194054 [zea mays]

7

gi|212721648

24

7.44

14

4.74

Not identified

Not identified

Not identified

412

GM

1279

Uncharacterized protein loc100383193 [zea mays]

17

gi|293336385

52

8.35

50

6.10

Not identified

Not identified

Not identified

739

Non-GM

325

Uncharacterized protein loc100502283 [zea mays]

5

gi|308080598

25

5.86

31

6.12

Not identified

Not identified

Not identified

789

GM

588

2-cys peroxiredoxin bas1 [zea mays]

8

gi|195626524

28

5.81

22

4.16

Apoplast, chloroplast

Antioxidant activity

Stress response

826

Non-GM

610

Apx1 - cytosolic ascorbate peroxidase [zea mays]

9

gi|226530305

27

5.55

27

5.92

Chloroplast, cell wall, plasma membrane

Antioxidant activity

Stress response

676

Non-GM

598

Glyoxylase1 [zea mays]

14

gi|162461576

32

5.59

35

5.87

Cytoplasm, nucleous

Gluthatione metabolism

Stress response

501

GM

701

Fructose-bisphosphate aldolase [Zea mays]*

10

gi|223975775

38

6.37

37

6.15

Cell wall, chloroplast, cytosol, mitochondrion, nucleolus, membrane

Glycolysis/gluconeogenesis

Carbohidrate metabolism

444

GM

548

Fructose-bisphosphate aldolase, cytoplasmic isozyme 1 [Zea mays]

6

gi|195612198

38

6.26

41

5.81

Cell wall, chloroplast, cytosol, mitochondrion, nucleolus, membrane

Glycolysis/gluconeogenesis

Carbohidrate metabolism

446

GM

883

Fructose-bisphosphate aldolase, cytoplasmic isozyme 1 [Zea mays]

9

gi|195612198

38

6.26

41

6.13

Cell wall, chloroplast, cytosol, mitochondrion, nucleolus, membrane

Glycolysis/gluconeogenesis

Carbohidrate metabolism

400

Non-GM

919

GADPH (383 AA) [Zea mays]

12

gi|22240

41

7.21

37

6.20

Chloroplast

Glycolysis/gluconeogenesis

Carbohidrate metabolism

427

GM

874

Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic-like [Brachypodium distachyon]*

13

gi|194688752

47

5.95

44

5.77

Chloroplast

Glycolysis/gluconeogenesis

Carbohidrate metabolism

555

GM

595

Lactoylglutathione lyase [Zea mays]*

8

gi|194701526

37

5.87

31

5.26

Chloroplast, peroxisome, plasma membrane, vacuole

Pyruvate metabolism

Carbohidrate metabolism

664

GM

368

H (+)-transporting ATP synthase [Zea mays]

7

gi|311237

20

4.35

24

4.37

Chloroplast

Photophosphorylation

Energy metabolism

656

2.1× Non-GM

947

Chaperonin [Zea mays]

13

gi|195623400

26

8.67

24

5.20

Apoplast, chloroplast, mitochondrion

Protein folding (chaperone)

Genetic information processing

286

Non-GM

424

S-adenosylmethionine synthetase 1 [Zea mays]*

8

gi|194689980

43

5.57

53

5.84

Cytoplasm

S-adenosylmethionine synthesis

Genetic information processing

320

Non-GM

429

Uncharacterized protein LOC100193051 [Zea mays]

5

gi|212723748

30

5.42

48

6.16

Not identified

Not identified

Not identified

515

Non-GM

500

Uncharacterized protein LOC100194161 [Zea mays]

4

gi|212721598

37

8.86

29

6.06

Not identified

Not identified

Not identified

690

2.6× GM

774

2-cys peroxiredoxin BAS1 [Zea mays]

14

gi|195626524

28

5.81

22

4.68

Chloroplast

Antioxidant activity

Stress response

618

GM

708

Apx2 - Cytosolic Ascorbate Peroxidase [Zea mays]*

10

gi|194707280

27

5.28

27

5.53

Chloroplast, cell wall, plasma membrane

Antioxidant activity

Stress response

557

Non-GM

326

IN2-1 protein [Zea mays]

3

gi|195612768

27

5.16

26

5.19

Chloroplast

Protein glutathionylation

Stress response

689

Non-GM

596

Pathogenesis-related protein 10 [Zea mays]

9

gi|226499304

17

5.13

18

5.21

Extracellular compartment

Defense response

Stress response

476

Non-GM

572

Peroxidase 42 precursor [Zea mays]*

8

gi|238011418

26

5.43

32

5.26

Extracellular compartment

Antioxidant activity

Stress response

  1. aProtein spot abundance: statistically significant values at P < 0.05. These protein spots are given in yellow boxes on Figure 3. bProtein name according to the best hit of MASCOT search against NCBI database. cFunctional protein classification according to the KEGG PATHWAY Database. Additional categories are included when necessary.