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Table 1 Proteins identified by ESI-Quad TOF and MALDI analysis

From: Proteins from Tuber magnatum Pico fruiting bodies naturally grown in different areas of Italy

ESI

Spot No. (a)

Acc. No. (b)

Organism

Protein Description

Score (c)

Seq. Cov. % (d)

Mw kDa Obs/Theo (e)

pI Obs/Theo (f)

Identified peptides (MS/MS) (g)

Tuber Gene annotation (h)

KOG Definition (i)

PFAM Definition (l)

Protein Blast relation (m)

1

D5G797

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_131, strain Mel28

104

4

78

46,2

6,2

5,82

2

GSTUM_00002523001

Aminoacylase ACY1

Peptidase M20

A

2

D5GJY5

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_55, strain Mel28

378

11

162

86,3

5,4

5,16

7

GSTUM_00009270001

Ultrahigh sulfur keratin-associated protein

Glyoxal oxidase

B

3

D5GGN7

T. melanosporum (Perigord truffle)

Dihydrolipoyl dehydrogenase

137

6

81

54

5,7

6,72

3

GSTUM_00007439001

Dihydrolipoamide dehydrogenase

Pyridine nucleotide-disulphide oxidoreductase

 

4

D5G8F0

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_15, strain Mel28

164

28

22,5

20

5,7

5,41

4

GSTUM_00004791001

Peptide methionine sulfoxide reductase

Peptide methionine sulfoxide reductase

C

5

D5GJ78

T. melanosporum (Perigord truffle)

S-adenosyl methionine synthase

871

47

57

41,8

5,8

5,7

15

GSTUM_00008874001

S-adenosylmeth. synthetase

S-adenosylmeth. synthetase

 
 

D5G9M7

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_169, strain Mel28

114

12

57

57,8

5,8

9,93

5

GSTUM_00003332001

RNA-binding protein

RNA recognition motif

D

6

D5GNA2

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_8, strain Mel28

125

22

22

22

5,5

6,21

4

GSTUM_00011192001

1,4-benzoquinone reductase

Flavodoxin

E

7

D5GAF9

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_18, strain Mel28

146

8

45

33

5,1

6,02

4

GSTUM_00005271001

Stationary phase-induced protein

SOR/SNZ family

F

8

D5GJY5

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_55, strain Mel28

170

9

91

86,3

5,4

5,16

6

GSTUM_00009270001

Ultrahigh sulfur keratin-associated protein

Glyoxal oxidase

 

9

D5GNA2

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_8, strain Mel28

1206

35

25

22

6,1

6,21

6

GSTUM_00011192001

1,4-benzoquinone reductase

Flavodoxin

G

11

D5G6L6

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_121, strain Mel28

118

21

41

31,3

5,2

6,01

5

GSTUM_00002097001

Predicted dehydrogenase

Short chain dehydrogenase

 

13

D5GC43

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_201, strain Mel28

149

18

38

33,5

5.1

5,06

5

GSTUM_00000555001

Lysophosphatidic acid acyltransferase

BAR domain protein

H

 

D5GAC6

T. melanosporum (Perigord truffle)

Probable Xaa-Pro aminopeptidase

131

5

38

69,2

5.1

5,28

3

GSTUM_00005237001

Xaa-Pro aminopeptidase

Peptidase_M24

 

14

D5GCN2

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_218, strain Mel28

83

22

19

20,7

5.4

8,64

3

GSTUM_00000743001

Alkyl hydroperoxide reductase/peroxiredoxin

Redoxin

I

15

D5G620

T. melanosporum (Perigord truffle)

Malate dehydrogenase

115

8

18

36,7

5.5

8,79

3

GSTUM_00001731001

NAD-dependent malate dehydrogenase

Lactate/malate dehydrogenase

 

16

D5GE86

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly scaffold_26, strain Mel28

105

14

28,2

26,8

5.5

7,1

4

GSTUM_00006427001

Predicted hydrolases of HD superfamily

No Hit Found

L

17

D5G5R4

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_112, strain Mel28

376

11

45,6

46,9

5.7

5,97

4

GSTUM_00001447001

Fructose 1,6-bisphosphate aldolase

Fructose-bisphosphate aldolase

M

 

Q1ACW3

Tuber borchii

NADP-dependent mannitol dehydrogenase

314

26

45,6

38

5.7

5,69

7

DQ223686.1

NAD-dependent malate dehydrogenase

NAD-dependent malate dehydrogenase

 

MALDI

Spot No. (a)

Acc. No. (b)

Organism

Protein Description

Score (c)

Seq. Cov. % (d)

Mw kDa Obs/Theo (e)

pI Obs/Theo (f)

Identified peptides (MS) (g)

Tuber Gene annotation (h)

KOG Definition (i)

PFAM Definition (l)

Protein Blast relation (m)

16

D5GJE0

T. melanosporum (Perigord truffle)

Whole genome shotgun sequence assembly, scaffold_50, strain Mel28

80

40%

28.2

25.6

5.5

5.77

11

GSTUM_00008955001

Alkyl hydroperoxide reductase

AhpC-TSA, AhpC/TSA family

 
  1. Table 1 List of proteins identified by ESI-Quad TOF and MALDI analysis. (a) as indicated in Figure 1; (b) UniProtKB accession number; (c) Mascot score; (d) percent sequence coverage; (e) gel-observed vs. theoretical molecular weights * Observed and theoretical weight may differ on the base of the real molecular weight of the protein in T. magnatum; (f) observed vs. theoretical isoelectric points; (g) number of matched peptides; (h) gene reference code; (i) and (l), gene model annotations (from http://mycor.nancy.inra.fr/IMGC/TuberGenome/Search.html); (m) numerical correlation to proteins obtained by blast analysis (Table 2). Spot 10 and Spot 12, no protein identification.
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