Figure 2

Two-dimensional gel electrophoresis analysis of gluten proteins during wheat grain development. A. Principal component analysis. Each symbol corresponds to the gluten protein gel pattern at a single developmental time point. The analysis includes the three replicas for each time point. For cluster d, the keys to the color spots correspond to the location of gel patterns above or below the origin of the y-axis. B-E. Representative 2-D gels of samples from clusters a, b, c, and d: MF-8, HF-6, MF-13 and MF-37 dpa. MN, MF, HN, and HF are defined in Table 1. Boxes in E outline positions of the HMW-GS, the ω-gliadins, and the LMW-GS plus α- and γ-gliadins. The proteome map and mass spectrometry data for identifications of individual proteins were reported previously [13]. Additional proteins identified in this study are indicated in panel B: AtpA, ATP synthase subunit alpha; CRT, calreticulin; Hsp70, 70 kD heat shock-induced protein; PDI, protein disulfide isomerase; R40, ABA- and stress-induced protein (Additional file 2 contains identification data).