Figure 5

Western blot analysis of adenylate kinase isoenzyme 1 [AK1 (spot 280)] and vinculin (spot 452). Figure 5 (Western blot) shows representative Western blots of AK1 (spot 280) and vinculin (spot 452). Four biological replicates were profiled at each of three timepoints. Numbers (1 to 4) at the bottom of the image indicate the four animals used in the experiment at each timepoint, each of which was run in an individual gel lane. Three technical replicates were run for each animal and the normalised value was used for statistical analysis. AK1 membranes show a gradual increment of band intensity with time post mortem. Vinculin blots show 4 bands in each lane that are changing across days post mortem. The graph shows the normalised average band density of vinculin for each band across the days post mortem and the average of all four bands normalised across the days post mortem. Band volumes which are significantly different (Tukey-Kramer analysis) are indicated with a and b. Figure 5 (2-D DIGE) shows representative bi- (A) and three- (B) dimensional expression profile of spots 452 (vinculin) and 280 (AK1) across three timepoints post mortem.