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Table 2 Biological function of the identified protein/fragment spots in porcine exudate

From: Monitoring post mortem changes in porcine muscle through 2-D DIGE proteome analysis of Longissimus muscle exudate

Spot a

UniProt b

Protein identified

Gene name

Biological process c

Peptides

pI d

MW (kDa) e

Score for g

13

Q08DP0

Phosphoglucomutase-1

PGM1

carbohydrate metabolic process

2

6.4

61.6

f20.16

13

Q3ZBZ8

Stress induced phosphoprotein 1

STIP1

immune system process; protein metabolic process; response to stress

3

6

62.4

f30.25

15

Q5E9A3

Poly(rC)-binding protein 1

PCBP1

neurological system process; intracellular protein transport; nuclear transport; induction of apoptosis; protein metabolic process; signal transduction

1

6.7

37.5

f10.15

21

P20072

Annexin A7

ANXA7

intracellular protein transport; signal transduction; lipid metabolic process; cell motion; signal transduction

1

6.4

50

f10.19

27

Q5XLD3

Creatine kinase M-type

CKM

muscle contraction; metabolic process

2

6.6

43.1

f20.21

35

Q8WZ42

Titin

TTN

assemblage and functioning of vertebrate striated muscles

2

6

3816.2

f20.15

47

Q7SIB7

Phosphoglycerate kinase 1

PGK1

carbohydrate metabolic process

2

8

44.4

f20.20

56

Q6S4N2

Heat shock protein 70

HSPA1B

immune system process; protein metabolic process; response to stress

20

5.6

70

g552

65

Q5D862

Filaggrin-2

FLG2

protein metabolic process; cellular component morphogenesis; ectoderm development

1

8.5

247.9

f10.15

78

P19378

Heat shock cognate 71

HSPA8

immune system process; protein metabolic process; response to stress

18

5.2

70.8

g290

86

P29700

Alpha-2-HS-glycoprotein (Fragment)

AHSG

immune system process; protein metabolic process; mesoderm development; skeletal system development

2

5.5

38.4

f20.32

119

Q29568

Phosphopyruvate hydratase (Fragment)

FH

glycolysis

2

4.6

16.1

f20.25

119

B1A3A0

Enolase

ENO3

glycolysis

2

8.1

47.1

f20.23

119

P19140

Alpha-enolase

ENO1

glycolysis

2

6.4

47.2

f20.17

122

Q5XKE0

Myosin-binding protein C, fast-type

Mybpc2

muscle contraction; intracellular protein transport; endocytosis; signal transduction; cell adhesion

2

6

127.3

f20.18

124

Q0VCY1

Vesicle-associated membrane protein-associated protein A

VAPA

membrane trafficking regulatory protein

2

8.9

27.8

f20.17

153

B1A3A0

Enolase

ENO3

glycolysis

2

8.1

47.1

f20.16

280

P00571

Adenylate kinase isoenzyme 1

AK1

nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

6

8.4

21.6

g136

358

Q06AB3

Ubiquitin carboxyl-terminal hydrolase isozyme L3

UCHL3

protein metabolic process

2

4.8

26.1

f20.27

452

P26234

Vinculin

VCL

cellular component morphogenesis

4

5.6

123.9

f40.17

462

Q2HJ54

Phosphatidylinositol transfer protein alpha isoform

PITPNA

visual perception; sensory perception; lipid transport; lipid metabolic process

2

6.1

31.8

f20.21

465

Q8TCA0

Leucine-rich repeat-containing protein 20

LRRC20

 

2

6.1

20.5

f20.28

552

P16419

Myosin-binding protein C, fast-type

Mybpc2

muscle contraction; intracellular protein transport; endocytosis; signal transduction; cell adhesion; protein metabolic process; cell motion

2

6.2

126.7

f20.19

565

Q9TSX9

Peroxiredoxin-6

PRDX6

immune system process; oxygen and reactive oxygen species metabolic process

15

5.7

25

g541

566

P34930

Heat shock 70 kDa protein 1A

HSPA1A

immune system process; protein metabolic process; response to stress

1

5.5

70

f10.15

566

A5A8V7

Heat shock 70 kDa protein 1-like

HSPA1L

immune system process; protein metabolic process; response to stress

1

6

70.3

f10.15

566

O97125

Heat shock protein 68

Hsp68

immune system process; protein metabolic process; response to stress

2

5.6

69.7

f20.18

566

P48720

Heat shock 70 kDa protein

HSPA1B

immune system process; protein metabolic process; response to stress

1

5.2

70.8

f10.16

566

P19120

Heat shock cognate 71

HSPA8

immune system process; protein metabolic process; response to stress

1

5.4

71.1

f10.16

566

Q8T869

Luminal-binding protein 2

bip2

 

1

5.1

70.4

f8.17

591

P08835

Serum albumin

ALB

transport

20

6.1

69.7

g504

591

Q710C4

Adenosylhomocysteinase

AHCY

nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

13

5.9

47.7

g116

595

P81605

Dermcidin

DCD

defense response

2

6.1

11.3

f20.15

648

Q3T0P6

cPhosphoglycerate kinase 1

PGK1

carbohydrate metabolic process

2

8.5

44.5

f20.28

680

Q08DP0

Phosphoglucomutase-1

PGM1

carbohydrate metabolic process

19

6.4

61.6

g294

962

Q9HC38

Glyoxalase domain-containing protein 4

GLOD4

metabolic process

2

5.4

34.8

f20.18

999

Q3SX44

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

DDAH2

mesoderm development; angiogenesis

2

5.7

29.8

f20.15

1007

P03974

Transitional endoplasmic reticulum ATPase

VCP

intracellular protein transport; exocytosis; protein metabolic process

2

5.1

89.2

f20.18

1011

A2THZ2

Albumin (Fragment)

ALB

transport

2

5.9

69.6

f20.17

1048

P52552

Peroxiredoxin-2 (Fragment)

PRDX2

immune system process; oxygen and reactive oxygen species metabolic process

7

4.7

14.2

g204

1054

Q0R678

DJ-1 protein

PARK7

immune system process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; protein metabolic process; response to stress

5

6.4

19.9

f50.21

1080

Q5E946

DJ-1 protein

PARK7

immune system process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; protein metabolic process; response to stress

10

6.8

20

g276

1100

Q1PC32

Triosephosphate isomerase (Fragment)

TPI

fatty acid biosynthesis; gluconeogenesis; glycolysis

2

6

21.8

f20.19

1100

Q3ZBZ8

Stress-induced-phosphoprotein 1

STIP1

immune system process; protein metabolic process; response to stress

2

6

62.4

f20.15

1135

Q1KYT0

Beta-enolase

ENO3

glycolysis

1

8.1

47

f10.17

1192

P34930

Heat shock 70 kDa protein 1A

HSPA1A

immune system process; protein metabolic process; response to stress

6

5.5

70

f58.25

1192

P08835

Serum albumin

ALB

transport

4

5.8

66.8

f40.18

1192

Q04967

Heat shock 70 kDa protein 6

HSPA6

immune system process; protein metabolic process; response to stress

1

5.8

71.1

f10.19

1220

Q8WZ42

Titin

TTN

assemblage and functioning of vertebrate striated muscles

2

6

3816.2

f20.16

1245

P69678

Protein CutA

CUTA

cation transport

2

8.6

19

f20.19

1291

A2THZ2

Albumin (Fragment)

ALB

transport

6

5.9

69.6

f60.27

1349

Q29371

Triosephosphate isomerase

TPI

fatty acid biosynthesis; gluconeogenesis; glycolysis

11

7.2

26.6

g476

  1. aSpot numbers refer to Figure 1. bAccession number in the UniProt database. cBiological process of the proteins obtained using PANTHER analysis [27]. dIsoelectric point of the protein. eMolecular weight of the protein. fTurboSEQUEST or gMASCOT score. These data are also available online in the 2-D PAGE reference protein map produced in our previous study under ‘Porcine Database’ [http://proteomics-portal.ucd.ie; Di Luca et al., [26].