Table 1 Summary of PhosSA, PhosphoRS and Sequest site assignment results for AQP2 mass spectrometry data set using CID and HCD fragmentations (see text for description). DNP denotes did not pass post-processing criteria
From: PhosSA: Fast and accurate phosphorylation site assignment algorithm for mass spectrometry data
CID data sets | PhosSA sensitivity(%) | PhosSA accuracy (%) | Sequest accuracy (%) | PhosphoRS accuracy(%) |
|---|---|---|---|---|
AQP2-H-(S256/S261) | 91.6 | 100 | 94.6 | 66.7 |
AQP2-M-(S256/S261) | 90.7 | 100 | 94.4 | 66.7 |
AQP2-L-(S256/S261) | 0 | DNP | 100 | 100 |
AQP2-H-(S264) | 91.5 | 100 | 100 | 100 |
AQP2-M-(S264) | 50 | 100 | 88.8 | 60 |
AQP2-L-(S264) | 0 | DNP | 60 | 50 |
HCD data sets | Â | |||
AQP2-H-(S256/S261) | 92.2 | 100 | 94.5 | 66.7 |
AQP2-M-(S256/S261) | 93.3 | 100 | 96 | 66.7 |
AQP2-L-(S256/S261) | 64.3 | 100 | 100 | 100 |
AQP2-H-(S264) | 97.7 | 100 | 74.2 | 75 |
AQP2-M-(S264) | 93.1 | 100 | 72.4 | 75 |
AQP2-L-(S264) | 0 | DNP | 50 | 50 |