Figure 2
From: Proteomic sample preparation for blast wound characterization
SDS-PAGE of effluent and serum samples after high abundant protein removal. Lane 1: Bio-Rad Precision Plus protein standard. Lanes 2 and 5: Crude effluent. Lanes 3 and 6: High abundant protein depleted effluent fraction. Lanes 4 and 7: Top-6 protein effluent fraction. Lanes 8 and 11: Crude serum. Lanes 9 and 12: High abundant protein depleted serum fraction. Lanes 10 and 13: Top-6 protein effluent fraction. Approximately 5 μg protein was loaded into each lane, proteins were visualized using SYPRO Ruby gel stain.