Figure 3
From: Proteomic sample preparation for blast wound characterization
High abundant protein removal improves spot number and resolution for wound effluent. Panel A shows crude effluent (Cy3/green) and high abundant protein depleted effluent (Cy5/red) overlayed spot maps from the same initial patient sample analyzed by 2-D DIGE. Panel B and C, respectively, represent crude effluent (Cy3) and high abundant protein depleted effluent (Cy5). This illustrates the Agilent Multiple affinity removal top-6 human column worked for wound effluent and greatly improved the overall spot number and quality.