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Figure 1 | Proteome Science

Figure 1

From: Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine

Figure 1

Two-dimensional gel electrophoresis maps of PNS prepared from control (A) and morphine-treated (B) rats. Protein samples (600 μg for both Silver and CBB staining) were separated in the first dimension on pH 3–11 IPG strips. For resolution in the second dimension, SDS-PAGE was performed in 10% w/v acrylamide/0.26% w/v bis-acrylamide gel. The stained 2D gels were scanned in an imaging densitometer and quantified by PDQuest software. The process of quantification of the difference between morphine-treated (+M10) and control (−M10) samples included spot detection, gel matching and spot quantification. Master gel was constructed for each group (+M10) or (−M10) as a synthetic image that contains the spot data from all the gels in the MatchSet. At least four replicates were performed for each group/sample. All matched and unmatched spots were then checked in a manual manner. Protein levels altered at least two-fold were taken into consideration. About 200 protein spots totally were recognized by CBB silver staining by PDQuest analysis. Proteins 1–10 with an altered mobility in (+M10) versus (−M10) samples were excised from in CBB-stained gels and identified by MALDI TOF/TOF analyzer as described in methods. Left panels, Silver staining; Right panels, CBB staining.

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