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Figure 3 | Proteome Science

Figure 3

From: Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine

Figure 3

Gβ subunit protein; immunoblot analysis of 2D-gels. A Two-dimensional resolution of Gβ protein content in PM isolated from control and morphine-adapted rats. PM protein (400 μg) was resolved by 2D electrophoresis using the pI range 3–11 for isoelectric focusing in the first dimension. The white small circle shows the small fraction of the the total signal of Gβ which was taken into consideration when analyzed by LC-MS/MS. The second dimension was performed by SDS-PAGE in 10% w/v acrylamide/0.26% bis-acrylamide gels (Hoefer SE 600). Gβ was identified by immunoblotting with specific antibody as described in Material and methods. The numbers 1–8 represent spots of Gβ subunits which were subsequently analyzed by LC-MS/MS. B The average of 3 immunoblots ± SEM. Difference between (−M10) and (+M10) was analyzed by Student’s t-test using GraphPadPrizm4 and found not significant, NS (p > 0.05).

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ISSN: 1477-5956