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Figure 1 | Proteome Science

Figure 1

From: Metabolic labeling with stable isotope nitrogen (15N) to follow amino acid and protein turnover of three plastid proteins in Chlamydomonas reinhardtii

Figure 1

Chlamydomonas reinhardtii growth is normal in TAP and 15 N-TAP but impaired in media containing D 2 O. Algal cell cultures were grown in each medium TAP, 15N-TAP and TAP containing each of the following: 30%, 60% and 90% D2O. Log phase algae grown in TAP (100 mL) were used to inoculate 6 mL of fresh media (TAP or labeled media) in 10 mL glass tubes. The tubes were shaken horizontally under fluorescent light (16:8) light:dark photoperiod) for synchronization of cell growth, and the OD600 determined twice daily by directly placing the tubes in a Spectronic 20 (Milton Roy Company) spectrophotometer. Each data point represents the mean of five replicates. The bars indicate the coefficient of variation.

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Proteome Science

ISSN: 1477-5956