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Figure 5 | Proteome Science

Figure 5

From: Metabolic labeling with stable isotope nitrogen (15N) to follow amino acid and protein turnover of three plastid proteins in Chlamydomonas reinhardtii

Figure 5

RuBisCo turnover example peptide (R)/DTDILAAFR/(M). Mass spectra showing degradation of old 15N labeled peptide (green) and emergence of newly synthesized partially labeled peptide (blue). Equal quantities of unlabeled peptide (red) were spiked into each sample to increase the success rate of peptide identification. The spike was 1:1 (unlabeled: time point) based on total protein concentration (Bradford assay). The old versus new peptide distributions are binomial or beta binomial distributions (old and new respectively) that are fitted to the data using maximum likelihood estimation. The ratio of the areas under the distributions provides relative quantities.

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ISSN: 1477-5956