Metabolic labeling with stable isotope nitrogen (15N) to follow amino acid and protein turnover of three plastid proteins in Chlamydomonas reinhardtii

Table 1 C. reinhardtii protein coverage

Protein	Accession #	Protein length	MW (kDa)	# aa from unique peptides	% coverage
RuBisCo	gi\|41179049	475	52.5	142	29.9
ATP synthase CF1 α subunit	gi\|41179050	508	54.8	219	43.1
ATP synthase CF1 β subunit	gi\|41179057	491	53.2	199	40.5
Mt F1F0 ATP synthase, α subunit	gi\|159483185	569	61.5	170	29.9
S-Adenosyl homocysteine hydrolase	gi\|159470383	483	52.7	144	29.8

Five proteins identified as co-resolving by SDS-PAGE at about 55 kDa. The band was excised from SDS-PAGE gels, the proteins extracted, tryptic digested and the samples analyzed by LC-MS/MS. Numbers of peptides were identified by Protein Pilot for each protein (Additional files 1 and 2).

ISSN: 1477-5956