Figure 2

UVB-induced loss of cell viability in CL1-0 and CL1-0ΔGR cells. MTT-based viability assays were performed where 5,000 CL1-0 and CL1-0∆GR cells were plated into 96-well plates in medium containing 10% FBS. After 24 h incubation, the cells were irradiated with the indicated doses of UVB for another 24 h. Cells were incubated with MTT and then DMSO added and the plates shaken for 20 min followed by measurement of the absorbance at 540 nm. Values were normalized against untreated samples and are the average of 4 independent measurements +/- the standard deviation (p < 0.05*, p < 0.001 ***).