Table 1 List of identified differentially expressed proteins between CL1-0 and CL1-0ΔGR in response to UVB-irradiation
Spot no. | Swiss-prot no. | Protein name | pI | MW | No. matched peptides | Cov. (%) | Scorea | CL1 + UVB/CL1b | ∆GR + UVB/∆GRb | Functional classification | Subcellular location |
|---|---|---|---|---|---|---|---|---|---|---|---|
1157 | P31946 | 14-3-3 protein beta/alpha | 4.76 | 28181 | 16/46 | 43% | 108/56 | -1.2 | -1.3 | Signal transduction | Cytoplasm |
962 | P29692 | Elongation factor 1-delta | 4.9 | 31219 | 9/40 | 32% | 77/56 | 1.11 | 1.46 | Protein biosynthesis | Cytoplasm |
1720 | P41567 | Eukaryotic translation initiation factor 1 | 6.89 | 12841 | 4/13 | 42% | 63/56 | -1.09 | 2.24 | Protein biosynthesis | Cytoplasm |
439 | Q16658 | Fascin | 6.84 | 55134 | 8/24 | 18% | 74/56 | 1.14 | 1.49 | Cell migration | Cytoplasm |
156 | P11142 | Heat shock cognate 71 kDa protein/HSC70 | 5.37 | 71086 | 10/30 | 19% | 76/56 | -1.06 | -1.3 | Protein folding | Cytoplasm |
127 | P08238 | Heat shock protein HSP 90-beta | 4.97 | 83560 | 9/22 | 16% | 68/56 | -1.15 | -1.34 | Protein folding | Cytoplasm |
207 | P20700 | Lamin-B1 | 5.11 | 66658 | 12/41 | 20% | 74/56 | -1.2 | -1.63 | Nuclear assembly | Nucleus |
1009 | Q15691 | Microtubule-associated protein RP/EB family member 1 | 5.02 | 30154 | 7/24 | 26% | 75/56 | 1.08 | 1.38 | Cell cycle | Cytoplasm |
228 | P38646 | Mortalin/GRP75/Stress-70 protein, mitochondrial | 5.87 | 73925 | 12/49 | 24% | 71/56 | 1.06 | 1.61 | Protein folding | Mitochondrion |
1433 | Q9UI09 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 | 9.63 | 17104 | 5/27 | 19% | 68/56 | -1.02 | -1.34 | Electron transport | Mitochondrion |
1521 | P23284 | Peptidyl-prolyl cis-trans isomerase B | 9.42 | 23786 | 10/48 | 46% | 87/56 | 1.05 | 1.72 | Protein folding | ER |
1361 | Q06830 | Peroxiredoxin-1 | 8.27 | 22328 | 8/40 | 32% | 81/56 | 1.13 | 1.54 | Redox regulation | Cytoplasm |
1352 | Q15102 | Platelet-activating factor acetylhydrolase IB subunit gamma | 6.33 | 25834 | 9/39 | 35% | 101/56 | 1.09 | 1.34 | Lipid degradation | Cytoplasm |
559 | P31153 | S-adenosylmethionine synthase isoform type-2 | 6.02 | 43981 | 9/32 | 28% | 85/56 | 1.14 | 1.46 | One-carbon metabolism | Cytoplasm |
939 | P37837 | Transaldolase | 6.36 | 37690 | 17/57 | 41% | 131/56 | -1.12 | -1.6 | Pentose phosphate pathway | Cytoplasm |
1209 | O43399 | Tumor protein D54 | 5.26 | 22282 | 8/32 | 48% | 90/56 | 1.12 | 1.34 | Cell proliferation | Cytoplasm |
1612 | P61088 | Ubiquitin-conjugating enzyme E2 N | 6.13 | 17185 | 6/33 | 35% | 64/56 | 1.19 | 1.87 | Protein degradation | Cytoplasm |
1104 | P21796 | Voltage-dependent anion-selective channel protein 1 (VDAC-1) | 8.62 | 30870 | 17/57 | 81% | 201/56 | -1.08 | 1.3 | Apoptosis | Mitochondrion |