Figure 1
From: Spatial proteomic and phospho-proteomic organization in three prototypical cell migration modes
Pseudopod purification system. (A) Cartoon of directional migration in response to different cues. Integrins, RTKs or GPCR can all induce directional cell migration. (B). Different experimental strategies to induce hapto- or chemo- taxis directional, versus control hapto- or chemo- kinesis random cell migration on 3 μm transwell filters. (C,D). Pseudopod extension dynamics on bottom filter surface. (C) Live imaging of pseudopod protrusion of bottom transwell surface. Serum-starved, Celltracker-labeled fibroblasts, were allowed to attach on the filter for one hour, stimulated with or not with chemoattractants and timelapse imaging was started for 3 hours. Bar = 50 μm. (D). Quantification of pseudopodial extension. Fluorescent intensity on the bottom filter was integrated and normalized to time point 0. Normalized signal indicates kinetics of membrane protrusion.