Figure 1

Proteomic analysis of laser capture microdissection of myotendinous junction regions. A) A comparative view of the myotendinous junction of a non-stained section (panel A) and an H&E stained section (panel B), with tendon and muscle regions identified as indicated and myotendinous regions identified by arrows. Panels C and D show a representative example of a myotendinous junction selected region (white dotted line) selected for microdissection before (panel C) and after (panel D) laser application and catapulting. All pictures taken with a 40X objective. Scale bar indicates 50 microns. B) Protein gel illustrating sections excised for LC-MS/MS analysis. The grid overlaying the lanes of solubilized material from myotendinous region (MTJ) or extrajunctional membrane (M) indicates the gel blocks used for in-gel tryptic digestions. C) A Venn diagram indicating the number of proteins identified according to their presence in the myotendinous junction (MTJ) or the extrajunctional membrane region (M).