Figure 3

Relationships between protein and mRNA expression levels of S100A10 and annexin A2. The expression levels of S100A10 and annexin A2 were examined by western blot analyses (A, B, D, E) and real-time qRT-PCR (C, D, E). (A) A western blot illustrating the differential expression of S100A10 and annexin A2 derived from cell lysates of 8 CRC cell lines. The results are representative of 3 separate experiments. S100A10 and annexin A2 protein expression levels in 8 CRC cell lines (B), S100A10 and annexin A2 mRNA expression levels in 13 CRC cell lines (C), mRNA and protein expression levels of S100A10 in 8 CRC cell lines (D), and mRNA and protein expression levels of annexin A2 in 8 CRC cell lines (E) were investigated by Pearson’s correlation coefficient test. There was a significant and positive correlation between S100A10 and annexin A2 mRNA expression levels (C), and S100A10 protein levels positively correlated with S100A10 mRNA levels (D). Data were plotted as the mean ± SD (n = 3). Western blot densitometry and relative quantity of S100A10 and annexin A2 transcripts are plotted as means ± SD (n = 3).