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Figure 1 | Proteome Science

Figure 1

From: Assessment of vascular endothelial growth factor in formalin fixed, paraffin embedded colon cancer specimens by means of a well-based reverse phase protein array

Figure 1

VEGF expression profiling by well-based reverse phase protein array and western blotting. We extracted total proteins from 2 different colon FFPE tissue specimens. (A) Western blotting shows different VEGF expression pattern. For reliable signal quantitation on same blot, we reprobed the membrane with anti-GAPDH antibodies. Quantative anlysis was performed using the ImageQuant program. (B) VEGF and GAPDH expressional signals were measured using well-based reverse phase protein array. Relative expressional signal of VEGF was displayed as a ratio of VEGF to GAPDH. Light bar and black bar represent results of western blotting and well-based reverse phase protein array (RPPA), respectively. The bar graph shows the average ± SD of two independent experiments.

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Proteome Science

ISSN: 1477-5956