Skip to main content
Figure 8 | Proteome Science

Figure 8

From: Pepsin-pancreatin protein hydrolysates from extruded amaranth inhibit markers of atherosclerosis in LPS-induced THP-1 macrophages-like human cells by reducing expression of proteins in LOX-1 signaling pathway

Figure 8

Confocal laser scanning microscopy depicting two-dimensional immunocytochemical localization of LOX-1 (A), ICAM-1 (C), MMP-9 (E) (green) and nuclei (blue) in human THP-1 macrophages after 24 h of treatment with extruded amaranth hydrolysates. Quantification of total LOX-1 (B), ICAM-1 (D) and MMP-9 (F) intensity over their respective area sums over treatment time (μm2). (A1) Nuclei C-; (A2) LOX-1 C-; (A3) Merge C-; (A4) Nuclei C+; (A5) LOX-1 C+; (A6) Merge C+; (A7) Nuclei treatment; (A8) LOX-1 treatment; (A9) Merge treatment. (C1) Nuclei C-; (C2) ICAM-1 C-; (C3) Merge C-; (C4) Nuclei C+; (C5) ICAM-1 C+; (C6) Merge C+; (C7) Nuclei treatment; (C8) ICAM-1 treatment; (C9) Merge treatment. (E1) Nuclei C-; (E2) MMP-9 C-; (E3) Merge C-; (E4) Nuclei C+; (E5) MMP-9 C+; (E6) Merge C+; (E7) Nuclei treatment; (E8) MMP-9 treatment; (E9) Merge treatment. Six independent fields of view from two independent cellular replicates were merged together per treatment group. Means with different letters are significantly different from each other (n = 2, p < 0.05).

Back to article page