Figure 2

Zoomed-in images of phosphorylated protein in different ontogenetic phases of apple seedling 02-18-081. The seedling 02-18-081 was derived from a cross hybrid ‘Jonathan’ × ‘Golden Delicious’, the proteins were extracted from leaf sample. Juvenile, adult vegetative and reproductive phases are designated by J, V and R, respectively. Panel a corresponds to global proteins stained with Coomassie Brilliant Blue R-350, panel b represents total phosphorylated proteins stained with Pro-Q Diamond, and panel c shows dephosphorylated proteins pre-treated with calf intestinal phosphatase and stained with Coomassie Brilliant Blue R-350. In this figure, spot numbers referenced in the text and in Table 1 (with their corresponding spots in parentheses) are A6-1 (spots 5298/4227/4965 in panel a; 1608/1706/2314 in panel b), A33-1 (4994/3929/- in panel a; 1632/1729/- in panel b), A33-2 (4998/3931/- in panel a; 1636/1734/- in panel b), A33-3 (4996/3927/4779 in panel a; 1635/1733/2421 in panel b), A48-1 (5026/4234/4791 in panel a; 1650/1760/2349 in panel b), A48-2 (5022/4236/4792 in panel a; 1651/1757/2351 in panel b), A48-3 (5027/4238/4796 in panel a; 1655/1758/2354 in panel b), B38-1 (4539/3595/4528 in panel a; 1733/1459/2191 in panel b), B38-2 (4540/3596/4529 in panel a; 1373/1456/2422 in panel b), B38-3 (4546/3593/4531 in panel a; 1379/1458/2424 in panel b), C24-1 (4471/4230/4460 in panel a; 1729/1399/2151 in panel b), C24-2 (4475/3551/4462 in panel a; 1330/1404/2152 in panel b), C24-3 (4478/3554/4465 in panel a; 1332/1406/2154 in panel b).