Protein phosphorylation differs significantly among ontogenetic phases in Malus seedlings

Table 1 Expression abundance of matched spots from different ontogenetic phases of three apple seedlings

02-18-081	A6-1	A33-1	A33-2	A33-3	A48-1	A48-2	A48-3	B38-1	B38-2	B38-3	C24-1	C24-2	C24-3	D1-1	D1-2	D1-3	D1-4	D146-1
J	0.061a	0.045b	0.045b	0.112b	0.030a	0.020b	0.025a	0.053a	0.381a	0.186a	0.010a	0.041a	0.095a	0.012a	0.006a	0.021a	0.028a	0.015a
V	0.063a	0.079a	0.057a	0.133a	0.022b	0.027a	0.018b	0.046b	0.302b	0.131b	0.008b	0.020b	0.039c	0.009ab	0.000	0.021a	0.018b	0.014a
R	0.040b	0.000	0.000	0.113b	0.017c	0.020ab	0.022ab	0.035c	0.157b	0.082b	0.007b	0.017b	0.073b	0.005b	0.000	0.028a	0.009c	0.016a
02-17-115	A6-1	A33-1	A33-2	A33-3	A48-1	A48-2	A48-3	B38-1	B38-2	B38-3	C24-1	C24-2	C24-3	D1-1	D1-2	D1-3	D1-4	D146-1
J	0.054a	0.000	0.000	0.098b	0.041a	0.040c	0.036a	0.063a	0.246a	0.165a	0.010a	0.039a	0.060a	0.000	0.000	0.046a	0.041a	0.066a
V	0.046b	0.000	0.044a	0.126a	0.015a	0.082a	0.028b	0.055b	0.196b	0.125b	0.015a	0.034a	0.094a	0.012a	0.014a	0.054a	0.027b	0.044b
R	0.037c	0.000	0.000	0.085b	0.000	0.066b	0.034a	0.046c	0.182b	0.138b	0.013a	0.027b	0.079a	0.000	0.000	0.031b	0.032b	0.025c
07-07-133	A6-1	A33-1	A33-2	A33-3	A48-1	A48-2	A48-3	B38-1	B38-2	B38-3	C24-1	C24-2	C24-3	D1-1	D1-2	D1-3	D1-4	D146-1
J	0.153a	0.182b	0.138ab	0.242b	0.139a	0.052b	0.098ab	0.175a	0.903a	0.661a	0.026a	0.030a	0.075a	0.018a	0.025a	0.083a	0.047b	0.103a
V	0.104b	0.260a	0.174a	0.341a	0.143a	0.080a	0.085b	0.128b	0.766b	0.560b	0.017b	0.026a	0.050ab	0.018ab	0.024a	0.100a	0.061a	0.083b
R	0.070c	0.211ab	0.145b	0.231b	0.091a	0.054b	0.118a	0.115b	0.693c	0.484c	0.000	0.000	0.042b	0.014b	0.018b	0.056b	0.035c	0.061c

J, juvenile phase; V, adult vegetative phase; R, reproductive phase. Data were from 2-DE gels containing global proteins. Statistical analysis using Student’s t-test at the p < 0.05 level was used to assess the significance of expression abundance differences among mutually matched gel spots from J, V and R phases within the same seedling.

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