Figure 1

Glucohexaose can induce ROS accumulation. A-B. Changes of O^2-(A) and H₂O₂(B) after treatment of glucohexaose. Cucumber cotyledons are infiltration with 0.1% NBT and 1 mg/mL DAB and decolourisation. One to fifteen hours after glucohexaose treatment indicated the plants’ early respond to glucohexaose. C-D. DPI and DMTU incubation can eliminate the ROS accumulation at five hours after glucohexaose treatment. E-J. Some important ROS scavenging enzymes activity in cucumber cotyledons with different treatment. CK represent the control group; P represent the cucumber cotyledons treated with 50 μg/mL glucohexaose for five hours; DPI and DMTU represent before treated with 50 μg/mL glucohexaose, DPI and DMTU were incubated for four hours. Error bar represent SD.