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Table 1 Glucohxaose and ROS modulators-regulated proteins

From: Proteomic analysis of Cucumis sativus cotyledons after glucohexaose treatment as a part of ROS accumulation related resistance mechanism

ID

gi

Protein name

PI

MW

Protein score

Protein score C.I. %

Pep. count

E-value

Fold enhancement by P6

Percentage of suppression by DPI/DMTU

Photosynthesis related proteins

          

1104

gi|12620881

Ribulose-1,5-bisphosphate carboxylase/oxygenase activase

5.54

48186.1

284

100

9

 

1.68

84.15/61.03

1111

gi|115768

Chlorophyll a-b binding protein of LHCII type I

5.14

27331.7

282

100

7

 

2.05

69.18/52.48

2004

gi|325515965

Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit

6.67

23461.8

651

100

12

 

7.76

50.81/24.37

4102

gi|255567170

Chlorophyll A/B binding protein

6.85

29362

82

99.482

5

 

1.40

82.39/55.08

0401

gi|62899808

Chromoplast-specific carotenoid-associated protein

5.05

35272.5

581

100

16

 

11.35

86.13/83.53

3507

gi|125578

Phosphoribulokinase

6.03

44485.6

552

100

12

 

2.97

73.35/77.00

3806

gi|12585325

Phosphoglucomutase

5.56

68625.8

322

100

8

 

2.36

48.99/41.42

5104

gi|11134156

Oxygen-evolving enhancer protein 2

8.61

28292.3

786

100

12

 

2.57

17.35/36.94

Metabolism-related proteins

          

1206

gi|225451299

Ribose-5-phosphate isomerase

6.66

30340.1

291

100

7

 

1.58

49.04/61.65

1606

gi|147838694

Chloroplast fructose-1,6-bisphosphatase

5.3

45183.4

898

100

11

 

3.55

88.59/33.13

2406

gi|3328122

Phosphoglycerate kinase precursor

7.68

50594

449

100

9

 

1.87

57.87/50.11

2702

gi|118721470

Vacuolar H + -ATPase subunit B

5.18

54450.9

575

100

17

 

4.21

81.96/47.07

5402

gi|307136265

Fructokinase

5.61

35800.6

403

100

16

 

2.15

68.33/52.23

7207

gi| 2833386

Ribulose-phosphate 3-epimerase

8.23

30632.2

299

100

5

 

9.18

79.61/78.13

7904

gi|1351856

Aconitate hydratase

5.74

98569.8

884

100

23

 

3.60

90.35/74.12

7905

gi|1351856

Aconitate hydratase

5.74

98569.8

1070

100

26

 

7.19

91.27/76.31

8402

gi|255557204

Fructose-bisphosphate aldolase, putative

7.59

38745.2

121

100

3

 

4.44

73.13/66.04

Chaperones and elongation factors

          

1813

gi|225445166

Elongation factor ts

4.78

123315.5

110

100

6

 

7.76

53.68/63.39

1804

gi|124245039

Chloroplast HSP70

5.18

75464.1

1070

100

28

 

15.05

99.55/86.18

1807

gi|124245039

Chloroplast HSP70

5.18

75464.1

1050

100

27

 

12.82

97.76/79.11

2808

gi|6911551

Heat shock protein 70

5.07

71843.3

570

100

25

 

5.65

95.26/76.54

3101

gi|255550363

Groes chaperonin

8.89

26582.2

73

95.384

2

 

17.20

25.06/28.38

3802

gi|402753

Translation elongation factor EF-G

5.04

77865.6

89

99.894

14

 

5.19

92.84/99.06

4202

gi|255550363

Groes chaperonin

8.89

26582.2

73

95.384

2

 

4.46

91.70/86.91

Peptidase enzymes

          

2807

gi|9759033

Acyl-peptide hydrolase-like

5.08

76116.9

160

100

10

 

5.33

92.74/65.70

2905

gi|297742722

Oligopeptidase B

5.21

79684.1

125

100

9

 

2.16

68.83/56.15

3807

gi|307136309

Serine-type endopeptidase

5.15

83277.2

145

100

13

 

2.55

84.32/37.22

3901

gi|297742722

Oligopeptidase B

5.21

79684.1

125

100

9

 

1.55

77.53/44.01

4801

gi|225468332

Similar to oligopeptidase A

5.61

58732.5

81

99.234

9

 

2.20

99.63/62.75

4802

gi|255572579

Oligopeptidase A, putative

5.71

88118.8

129

100

13

 

2.13

99.48/44.81

4903

gi|255537515

Aminopeptidase, putative

6.04

98135.3

131

100

15

 

11.40

98.35/65.03

5902

gi|255537515

Aminopeptidase, putative

6.04

98135.3

304

100

15

 

10.90

98.02/46.32

5903

gi|25083482

Putative aminopeptidase

5.43

99495.2

252

100

15

 

15.04

98.38/77.61

5904

gi|255537515

Aminopeptidase, putative

6.04

98135.3

250

100

18

 

13.49

99.19/50.26

Protein phosphatase

          

0303

gi|15240071

Putative protein phosphatase 2C 80

7.6

44302

72

94.7

3

 

2.46

29.15/49.70

Antioxidation proteins

          

1402

gi|18874402

Galactinol synthase

4.81

38608

130

100

5

 

7.62

93.74/14.20

1802

gi|11559422

Disulfide isomerase

5.07

37249

589

100

20

 

3.84

89.41/74.09

1803

gi|11559422

Disulfide isomerase

5.07

37249

589

100

20

 

3.38

85.47/57.64

1805

gi|11559422

Disulfide isomerase

5.07

37249

589

100

20

 

3.01

91.93/51.20

1806

gi|11559422

Disulfide isomerase

5.07

37249

589

100

20

 

3.47

94.47/68.43

2105

gi|240252434

NifS-like protein

6.24

67894.1

69

89.179

10

 

4.07

11.20/34.32

2107

gi|297842615

Glutathione S-transferase

5.76

75307.3

101

99.993

8

 

2.22

67.69/9.46

3808

gi|341579690

Betaine-aldehyde dehydrogenase

5.25

55339.3

602

100

16

 

4.58

77.64/59.24

5804

gi|124057819

Raffinose synthase

5.42

87904.5

96

99.975

17

 

391.29

99.03/60.22

7204

gi|117663160

Carbonic anhydrase

6.3

10976.5

175

100

5

 

225.27

80.50/99.93

7302

gi|15222954

Thioredoxin-like protein CDSP32

8.65

33948.5

253

100

8

 

1.59

26.05/52.03

8202

gi|117663160

Carbonic anhydrase

6.3

10976.5

257

100

7

 

6.96

71.19/80.24

Others

          

3803

gi|224065421

Peptidylprolyl isomerase

5.03

64379.7

105

99.997

9

 

5.19

94.72/75.20

5703

gi|108710583

Adenylosuccinate synthetase

9.07

51473.6

247

100

7

 

3.04

70.41/61.06

5711

gi|255578102

Imidazole glycerol phosphate synthase subunit hisf

6.62

65225.3

496

100

16

 

1.88

55.65/64.14

5803

gi|9759324

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

5.89

80394.2

700

100

25

 

3.81

99.30/74.98

5812

gi|225448296

PREDICTED: hypothetical protein

6.55

106446.4

183

100

10

 

1175.24

75.18/83.99

7406

gi|18401429

N-carbamoylputrescine amidase

5.71

33683

242

100

6

 

6.86

92.78/63.85

7606

gi|255562088

Transaminase mtnE

6.95

50909.2

346

100

10

 

1.77

54.52/42.84