Figure 3

Summary of the protein expression data in colorectal tumors vs corresponding spheroids. (A) Venn diagrams illustrating overlapping sets of unique proteins identified in CRC tissues and CRC spheroids derived thereof. (B) Tables denoting the number of differential proteins, respectively, with different criteria for filtering. Abbreviations: CRC, colorectal tumor tissue; SPH, CRC spheroids; FC, fold change; Sp. Count separated, number of proteins from the third column (p < 0.05 & FC > 2) that, in addition, exhibit spectral count separation: the lowest spectral count in one group is higher than the highest spectral count in the other group. (C) Heat map view of a supervised cluster analysis using differential proteins from the comparison CRC tissue and spheroid proteomes (174 proteins with 1.5 fold change in all 3 pairs). The protein abundances were normalized to zero mean and unit variance for each protein. Two clusters are formed for the CRC tissue lysate and spheroid groups. CRC, colorectal cancer; tissue sample; SPH, spheroid sample.