Figure 6

Network of down-regulated proteins (referenced by gene symbols) in all CRC-SPH pairs. Proteins down-regulated with a fold change > 1.5 in all three pairs of spheroids (SPH) versus their parent colorectal carcinoma tissue (CRC) were uploaded to STRING, and the returned protein-protein link data were used to generate and annotate a protein network in Cytoscape. Nodes and their labels were sized according to the number of edges connected to them. Node color was made dependent on the overall fold change in all three CRC-SPH pairs: nodes exhibiting a defined fold change were colored along a white-to-green gradient (minimum and maximum value indicated for connected nodes), whereas nodes only detected in SPH were colored black, see color legend. The unconnected PLIN2 node has a fold change of 24, but was given the color of the top of the gradient.