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Figure 1 | Proteome Science

Figure 1

From: An integrative strategy for quantitative analysis of the N-glycoproteome in complex biological samples

Figure 1

Flow chart of the integrated strategy. Serum samples were digested with trypsin after removal of high-abundance proteins. Glycopeptides were enriched with lectin chromatography from two different samples, desalted, and then catalyzed by immobilized trypsin and PNGase-F in 18O or 16O water, as indicated. Equal amounts of 18O- and 16O-labeled glycopeptides were mixed, and the 6 Da mass shifts were generated between paired, labeled glycopeptides, which could be identified by subsequent LC-ESI-MS/MS. The data were quantitatively analyzed automatically with the self-build quantitative method.

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