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Figure 2 | Proteome Science

Figure 2

From: An integrative strategy for quantitative analysis of the N-glycoproteome in complex biological samples

Figure 2

Linearity and accuracy of the self-build quantitative method in glycopeptides from glycoprotein standards. Four glycopeptides, FATN*TTLTK and LMTN*ETSDRPLVHFTPNK from invertase [Swiss-Prot:P00724] and AESN*GSYLQLVEISR and LAPLN*DSR from Fetuin [Swiss-Prot:P12763], were serially diluted to determine the linearity and accuracy of the self-build quantitative method. On the left are the results from manual calculation and on the right are the results from the self-build quantitative method. Glycopeptide concentration ratios calculated manually and those from the self-build method were all significantly associated with the theoretical values, with both correlation coefficients (R2) being >0.99. *denotes the N-glycosylation site.

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