Figure 4

Pull-down experiments applying probe 1 to cell lysates derived from Colo205 cells treated with pervanadate or water as the control (triplicates). A: Volcano plot of the measured differences using intensity based label-free quantification and B: SH2 protein enrichment indicated by the SH2-protein LFQ intensity ratio from the MS experiments, displayed are medians of the intensity ratios of the pervanadate and water treated samples from the triplicate analysis. The error bars display the standard deviation between the replicates. The comparably high standard deviation in the pervanadate treated samples mainly originates from one protein in one of the replicates (for detailed data refer to supplementary information).