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Table 2 Summarized results of three iTRAQ experiments

From: Plasma protein profiling of Mild Cognitive Impairment and Alzheimer’s disease using iTRAQ quantitative proteomics

Biological replicate experiment Unused* (Conf) cutoff Number of unique proteins detected Number of proteins before grouping Number of distinct peptides identified Number of spectra identified % Total spectra ¶
iTRAQ 1 >1.3 (95) 93 352 5047 11908 72.1
iTRAQ 2 >1.3 (95) 107 357 7614 17211 76.4
iTRAQ 3 >1.3 (95) 96 301 6392 15789 71.8
  1. *A ProteinPilot Unused score of >1.3 indicates >95% confidence in correct protein sequence identification.
  2. ¶Spectra identified ÷ total MS/MS spectra.