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Table 2 Summarized results of three iTRAQ experiments

From: Plasma protein profiling of Mild Cognitive Impairment and Alzheimer’s disease using iTRAQ quantitative proteomics

Biological replicate experiment

Unused* (Conf) cutoff

Number of unique proteins detected

Number of proteins before grouping

Number of distinct peptides identified

Number of spectra identified

% Total spectra ¶

iTRAQ 1

>1.3 (95)

93

352

5047

11908

72.1

iTRAQ 2

>1.3 (95)

107

357

7614

17211

76.4

iTRAQ 3

>1.3 (95)

96

301

6392

15789

71.8

  1. *A ProteinPilot Unused score of >1.3 indicates >95% confidence in correct protein sequence identification.
  2. ¶Spectra identified ÷ total MS/MS spectra.