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Table 1 Proteins identified and analyzed by MALDI-TOF-TOF/MS

From: Screening and identification of resistance related proteins from apple leaves inoculated with Marssonina coronaria (EII. & J. J. Davis)

Spot no

Protein name

Accession no.

Deduced apple genome ID

Protein MW/PI

Pep. count

Protein score

Relative protein content

Photosynthesis proteins

L8

ribulose-1,5-bisphosphate carboxylase

gi|2961315

MDP0000597996

53314.4/6.14

5

253

L45

ribulose-1,5-bisphosphate carboxylase/oxygenase activase alpha 2

gi|78100212

MDP0000944409

46944.1/4.84

12

494

L49

ribulose-1,5-bisphosphate carboxylase/oxygenase activase small protein isoform

gi|115334979

MDP0000321244

47952.1/7.57

10

326

L50

Oxygen-evolving enhancer protein 2, chloroplastic; Short = OEE2;

gi|11134051

MDP0000361338

28674.3/6.6

4

231

L51

ribulose 1,5-bisphosphate carboxylase

gi|4206530

MDP0000597996

49888.8/5.94

3

223

L82

ribulose 1,5-bisphosphate carboxylase

gi|10945633

MDP0000597996

52133/6.18

8

154

L85

ribulose-phosphate 4-epimerase

gi|225457361

MDP0000137234

30146/8.93

5

533

L95

ribulose 1,5-bisphosphate carboxylase

gi|371928199

MDP0000597996

49673/6.04

20

498

L15

Chlorophyll a-b binding protein 3C-like protein

gi|357497757

MDP0000784451

24884.4/5.53

4

133

L19

putative chlorophyll a/b binding protein

gi|397789264

MDP0000875642

16135/7.82

6

262

L61

Chlorophyll a-b binding protein 2, chloroplastic;

gi|158562858

MDP0000708928

3798.9/8.2

4

212

L87

chlorophyll a-b binding protein 8, chloroplastic

gi|225436257

MDP0000866655

29511.2/7.85

2

192

Amino acid metabolism related proteins

L43

Serine-type peptidase

gi|357495999

MDP000065459

45945.4/6.79

9

267

L54

precursor of carboxylase p-protein 1, glycine decarboxylase complex

gi|224088838

MDP0000588069

115985/6.51

11

407

L58

putative plastidic glutamine synthetase

gi|26892040

MDP0000139493

47619.1/6.77

9

457

Transpot related proteins

L56

Transketolase, chloroplastic

gi|75140229

MDP0000142098

73346.7/5.47

6

20-6

L81

alanine aminotransferase 3

gi|351724777

MDP0000815368

53789.3/5.52

11

470

L99

PREDICTED: transketolase, chloroplastic-like

gi|356536526

MDP0000142098

80689.8/6.12

11

299

L101

Transketolase

gi|357445031

MDP0000142098

80087.5/6.00

10

219

Energy metabolism related proteins

L23

PREDICTED: ferredoxin--NADP reductase, leaf isozyme, chloroplastic-like isoform 1

gi|356538289

MDP0000811918

40803.8/8.7

14

477

L24

NAD dependent epimerase/dehydratase

gi|255559448

MDP000019372

43230.8/8.75

6

243

Carbohydrate metabolism related proteins

L27

PREDICTED: triosephosphate isomerase

gi|225449541

MDP0000152242

27396.2/6.34

5

373

L33

alcohol dehydrogenase

gi|307135978

MDP0000239956

40657.6/8.53

3

138

L36

cytosolic malate dehydrogenase

gi|78216493

MDP0000174740

35970.5/6.01

3

352

L40

glyceraldehyde-3-phosphate dehydrogenase A

gi|381393060

MDP0000527995

43224.5/8.1

18

853

L41

PREDICTED: fructose-1,6-bisphosphatase, cytosolic

gi|225460680

MDP0000251810

37554/6.03

7

332

L42

glyceraldehyde-3-phosphate dehydrogenase B

gi|381393062

MDP0000835914

48490.9/8

21

790

L46

glyceraldehyde 3-phosphate dehydrogenase A subunit

gi|62318887

MDP0000527995 c

17730.1/5.35

6

210

L47

NADP-dependent glyceraldehyde-3-phosphate dehydrogenase;

gi|3913711

MDP0000152497

53654.6/6.76

10

375

L53

alcohol dehydrogenase

gi|307135978

MDP0000239956

40657.6/8.53

8

216

L59

chloroplast sedoheptulose-1,7-bisphosphatase

gi|118175929

MDP0000244771

42824.9/6.06

9

377

L83

PREDICTED: triosephosphate isomerase

gi|225449541

MDP0000152242

27396.2/6.34

6

266

L86

PREDICTED: pyruvate dehydrogenase E1 component subunit beta

gi|357159289

MDP0000146411

40081.6/5.47

5

407

Binding proteins

L68

ATP synthase, subunit B

gi|7578491

MDP0000565338

53365/5.57

15

752

L69

ATP synthase subunit B

gi|91983091

MDP0000565338

51812.1/5.27

21

703

L71

ATP synthase, subunit B

gi|7578491

MDP0000565338

53365/5.57

19

791

L72

putative ATP synthase subunit B

gi|56784991

MDP0000565338

45936.9/5.33

17

971

L73

PREDICTED: ATP synthase subunit B, mitochondrial-like

gi|225424142

MDP0000565338

59699.2/5.84

18

760

L77

ATP synthase, subunit B

gi|7578491

MDP0000565338

53365/5.57

19

632

L78

ATP synthase CF1 alpha subunit

gi|346683279

MDP000092905

55443.9/5.09

21

1100

L79

ATP synthase subunit B

gi|91983091

MDP0000565338

51812.1/5.27

24

923

Antioxidant related proteins

L25

Probable aldo-keto reductase 2

gi|378548276

MDP0000228499

38436.6/6.17

6

341

L37

aldo/keto reductase AKR

gi|62526573

MDP0000228499

38026.5/6.38

10

483

Defense and stress related proteins

L13

class III endo-chitinase

gi|33347391

MDP0000280265

17774.6/4.12

1

207

L16

heat shock protein 70

gi|30962610

MDP0000322220

47584/5.1

9

106

L17

beta-1,3-glucanase

gi|399137110

MDP0000570395

37551.2/5.07

4

258

L18

thioredoxin peroxidase

gi|21912927

MDP000020081

30103.4/8.2

5

328

L22

thaumatine-like protein

gi|20149274

MDP0000552328

22948.5/4.6

7

317

L28

ascorbate peroxidase

gi|145581388

MDP0000199034

27711.8/5.53

6

414

L29

ascorbate peroxidase

gi|319993039

MDP0000194474

12064.3/8.72

3

73

L30

early-responsive to dehydration 2

gi|383100964

MDP0000322220

69263.1/5.19

11

149

L84

quinone oxidoreductase [Gymnadenia conopsea]

gi|89276317

MDP0000393227

21631.1/6.06

2

159

L89

phi class glutathione S-transferase protein

gi|329130898

MDP0000266097

23996.3/5.97

3

90

L90

IgE-binding protein MnSOD

gi|10862818

MDP0000187714

22957.7/6.06

6

397

Unknown proteins

L20

uncharacterized protein LOC100805310

gi|359806638

MDP0000875642

27959.1/5.29

9

353

L31

PREDICTED: uncharacterized protein At2g37660, chloroplastic

gi|225440390

MDP0000176370

27681.5/5.85

2

150

L32

predicted protein

gi|224087915

MDP0000641719

27403.9/5.74

9

464

L38

PREDICTED: clavaminate synthase-like protein At3g21360

gi|225442460

MDP0000406399

36588.7/6.1

4

80

L57

predicted protein

gi|224138316

MDP0000148186

45396.1/6.11

14

534

  1. *In relative protein content graph, from left to right were : Control group 3 days post-treatment, : Inoculated group 3 days post-treatment, : Control group 6 days post-treatment, : Inoculated group, 6 days post-treatment respectively.