Skip to main content

Table 2 Gene-specific primer pairs used for qRT-PCR analysis of four identified genes in ‘Qinguan’ leaves inoculated with M. coronaria

From: Screening and identification of resistance related proteins from apple leaves inoculated with Marssonina coronaria (EII. & J. J. Davis)

Spot no.

Gene name

Accession no.

Primer sequence (5’-3’)

L13

Class III endo-chitinase

gi|33347391

Forward: GCACTCAACGGACACAAC

   

Reverse: GTAGAACTGAACCCAAACG

L17

Beta-1, 3-glucanase

gi|399137110

Forward: AGTCGTATCGGAGAGTGGTTG

   

Reverse: TGAGTTGGTACTTGGGTTGTTT

L22

Thaumatine-like protein

gi|20149274

Forward: ATGGTCTGGTCGCTTCTG

   

Reverse: CCGTCAACAAGGCTAACA

L28

Ascorbate peroxidase

gi|145581388

Forward: AAGGTGCCACAAGGAGCG

   

Reverse: AGAGGGCGGAAGACAGGG